2D PAGE: Sample Preparation and Fractionation: Volume 2 (Methods in Molecular Biology) [Hardcover]

Anton Posch (Editor)

Book Description

ISBN-10: 1603272097 | ISBN-13: 978-1603272094 | Publication Date: March 19, 2008 | Edition: 1

Focusing on two-dimensional electrophoresis (2-DE), this book sets forth the principles and recent developments of sample preparation and fractionation tools in expression proteomics. Step-by-step instructions alongside detailed illustrations enable you to quickly learn how to perform the methods yourself. Tricks of the trade and notes on troubleshooting and avoiding pitfalls help you ensure successful results every time. The methods covered in this book are not limited to 2-DE; they can also be applied to a broad range of protein separation techniques.

Editorial Reviews

From the Back Cover

The complex nature of proteins often requires a multitude of sample preparation options. In 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2, Dr. Anton Posch presents a broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics and specifically for two-dimensional electrophoresis (2-DE). Detailed laboratory methods describe the process of sample fractionation used to reduce the complexity of the protein sample and the methods of 2-DE analysis and a broad variety of gel-free mass spectrometry-based technologies to detect previously undetectable proteins. Vital information regarding protein isolation and preparation for reproducible proteome analysis previously overlooked by scientists has been clearly laid out in this pioneering two-volume set. Volume 2 illuminates specific applications of sample preparation tools and fractionation strategies to study a variety of biological systems.   Invaluable for students of Biochemistry, Biomedicine, Biology and Genomics and essential for the practicing scientists, 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2 delves into the study of proteins with comprehensive and in depth methods and protocols to provide a greater understanding of this intricate and complicated field.

Product Details

• Hardcover: 353 pages
• Publisher: Humana Press; 1 edition (March 19, 2008)
• Language: English
• ISBN-10: 1603272097
• ISBN-13: 978-1603272094
• Product Dimensions: 9.3 x 6.3 x 0.9 inches
• Shipping Weight: 1.7 pounds (View shipping rates and policies)
• Amazon Best Sellers Rank: #3,661,667 in Books (See Top 100 in Books)