This is a collection of affinity-based purification protocols with an international authorship from across the life sciences. The introductory chapter outlines recent advances in ligand-coupling, matrix choice, and operating modes. The second chapter describes methods for obtaining quantitative data from affinity processes. There are specific chapters describing methods for the affinity separation of proteins, nucleic acids, and oligosaccharides. Methods for immunoglobulin purification and immunoaffinity protocols are discussed in a further chapter, and the use of affinity tags in the recovery of engineered proteins in another. Finally, modern techniques for cell separations are outlined. The book presents detailed laboratory protocols together with troubleshooting advice and reviews of recent developments. This book is aimed at bench scientists, both those in analytical research laboratories, and those non-specialists looking to apply affinity methods in their work; postgraduate and post-doctoral researchers, and academic staff in analytical research and general bioscience laboratories.
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Book Description
Editorial Reviews
Review
"While the editor suggests that it is not intended as a textbook, this book would certainly provide a valuable additional source for courses on bioseparations. However, its most welcome reception will come from laboratory scientists at all levels..."--Journal of Medicinal Chemistry
"This publication is designed to provide novice laboratory technicians and researchers with the background and examples necessary to develop and apply an affinity purification scheme. Built around practical examples, the chapters provide step-by-step protocols for the affinity separation of proteins, nucleic acids, oligosaccharides, immunoglobulin purification, and immunoaffinity applications. The eight individually authored chapters are further enhanced with background and troubleshooting information as well as charts, graphs, example results, and references." -- Biosis, Vol 50, Issue 10, Oct 22, 1998
--This text refers to the Paperback edition.
About the Author
Paul Matejtschuk, Doctor, Research and Development, BioProducts Laboratory, Hertfordshire.
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First Sentence:
Since the innovative work of Porath et al. (1) in the 1960s, and the seminal texts of Lowe and Dean (2) and Dean et al. (3), the technologies of affinity separation have become widely accepted-perhaps now to the extent of being taken for granted. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
aminated support, boronate matrix, alternating histidines, recycling partition technique, weak affinity chromatography, quantitative affinity chromatography, boronate ligands, frontal affinity chromatography, agarose purification, hapten sugars, immunoaffinity separations, biosensor response, ligand leakage, partitioning solute, bound solute, equilibration buffer, immobilized lectin, activated gel, abscissa intercept, polymerase domain, biosensor chip, tresyl chloride, immunomagnetic beads, eluting buffer, purification cycle Key Phrases - Capitalized Phrases (CAPs): (learn more)
New York, Academic Press, Cibacron Blue, Fast Flow, Con A-Sepharose, Natl Acad, Chelating Sepharose, Phenyl Sepharose, Protein Expr, Bioprocessing Ltd, Trends Biotechnol, Nucleic Acids Res, Elsevier Applied Science, Plenum Press, Marcel Dekker, Royal Society of Chemistry, Staphylococcus Protein, Stockton Press New!
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Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Since the innovative work of Porath et al. (1) in the 1960s, and the seminal texts of Lowe and Dean (2) and Dean et al. (3), the technologies of affinity separation have become widely accepted-perhaps now to the extent of being taken for granted. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
aminated support, boronate matrix, alternating histidines, recycling partition technique, weak affinity chromatography, quantitative affinity chromatography, boronate ligands, frontal affinity chromatography, agarose purification, hapten sugars, immunoaffinity separations, biosensor response, ligand leakage, partitioning solute, bound solute, equilibration buffer, immobilized lectin, activated gel, abscissa intercept, polymerase domain, biosensor chip, tresyl chloride, immunomagnetic beads, eluting buffer, purification cycle Key Phrases - Capitalized Phrases (CAPs): (learn more)
New York, Academic Press, Cibacron Blue, Fast Flow, Con A-Sepharose, Natl Acad, Chelating Sepharose, Phenyl Sepharose, Protein Expr, Bioprocessing Ltd, Trends Biotechnol, Nucleic Acids Res, Elsevier Applied Science, Plenum Press, Marcel Dekker, Royal Society of Chemistry, Staphylococcus Protein, Stockton Press New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
This book cites 2
books:
2
books
cite this book:
- The United States (Handbooks to the Modern World, 3-Vol. Set) by Godfrey Hodgson on page 194
- Affinity Chromatography by Christopher Robin Lowe on page 35
- Handbook of Bioseparations, Volume 2 (Separation Science and Technology) by Satinder Ahuja on page 630
- Quantitative Characterization of Ligand Binding by Donald J. Winzor in Back Matter
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