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Agrobacterium Protocols: Volume II (Methods in Molecular Biology) [Hardcover]

Kan Wang
5.0 out of 5 stars  See all reviews (1 customer review)

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Book Description

September 1, 2006 1588298434 978-1588298430 2nd
Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agric- tural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger “gene silencing” often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Ag- bacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent devel- ments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.

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Agrobacterium Protocols: Volume II (Methods in Molecular Biology) + Agrobacterium Protocols: Volume I (Methods in Molecular Biology)
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Editorial Reviews

Review

From the reviews of the second edition:

"This is, as the title suggests, a laboratory manual with protocols for using Agrobacterium tumefaciens to transform plant species … . the book will be a valuable addition to the laboratory bookshelf. The protocols … are clearly set out and the notes explain things … . I was also pleased that some chapters mentioned the need to consider regulatory issues regarding disposal of genetically modified materials, something that is often ignored in lab manuals." (Heather Macdonald, SGM – Society for General Microbiology, 2007)


Product Details

  • Series: Methods in Molecular Biology (Book 344)
  • Hardcover: 512 pages
  • Publisher: Humana Press; 2nd edition (September 1, 2006)
  • Language: English
  • ISBN-10: 1588298434
  • ISBN-13: 978-1588298430
  • Product Dimensions: 1.3 x 6.2 x 9 inches
  • Shipping Weight: 2 pounds (View shipping rates and policies)
  • Average Customer Review: 5.0 out of 5 stars  See all reviews (1 customer review)
  • Amazon Best Sellers Rank: #2,484,932 in Books (See Top 100 in Books)

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5.0 out of 5 stars eduaruto March 4, 2012
Format:Hardcover|Verified Purchase
GREAT ATENTION!!! EVEN PERSONALIZED!!! IF YOU NEED IT, THEY WILL CALL YOU TO HELP YOU WITH ANY PROBLEM ABOUT YOUR ITEM!!! STRONGLY RECOMENDED!!! FOR SURE I'LL BY FROM HERE AGAIN!!!
AND BEAUTIFULL VOICE OF THE GIRL CALLING TO HELP YOU HAHA
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