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Antibody Applications: Essential Techniques [Spiral-bound]

Peter J. Delves (Author)


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Book Description

November 21, 1995 0471956988 978-0471956983 1
Antibodies have become one of the most important tools in the areas of cell and molecular biology. Antibodies offer a unique combination of sensitivity and specificity that surpasses nearly all other detection systems. Hence immunoassays of all types are now widely used in many areas of research. This book describes in clear, very precise protocols the whole range of important immunodetection methods in current use in cell and molecular biology and as such it will be essential for anyone either using or about to use antibody techniques in their work. The Essential Techniques Series books are designed to provide you with immediate access to the protocols you require every day. These handy pocket-sized manuals are easy to carry around, and conveniently spiral bound making them ideal for lab bench work. Written by experienced laboratory researchers, each book in the Essential Techniques Series gives up-to-date, tried and tested practical information for the life scientist. For each key technique these books:
  • introduce the most commonly used methods,
  • explain the advantages and disadvantages of the methods, and give advice on which procedure to use,
  • provide easy to follow step-by-step protocols, with experimental notes and tips on where to pause, plus information on safety and suppliers.

Editorial Reviews

From the Publisher

A handy, spiral-bound, laboratory manual that permits quick and easy access to the major protocols essential to perform antibody applications. Lists 38 procedures and includes practical tips to indicate hazards, to stop a method, key conditions, reagent disposal plus a troubleshooting guide.

Product Details

  • Spiral-bound: 164 pages
  • Publisher: Wiley; 1 edition (November 21, 1995)
  • Language: English
  • ISBN-10: 0471956988
  • ISBN-13: 978-0471956983
  • Product Dimensions: 8.4 x 5.5 x 0.6 inches
  • Shipping Weight: 9 ounces
  • Amazon Best Sellers Rank: #5,354,909 in Books (See Top 100 in Books)

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Inside This Book (learn more)
First Sentence:
Antibodies secreted in vivo in response to immunization with antigen are usually polyclonal because several different B-cells will be stimulated which recognize different epitopes on the immunogen. Read the first page
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
residual buffer, cold buffer, primary antibody, tissue sections
Key Phrases - Capitalized Phrases (CAPs): (learn more)
New York, Methods Enzymol, Reagents Antibody, Practical Approach, Scientific Publishers, Technique Equipment Absorbent, Academic Press, Microcentrifuge Microcentrifuge, Natl Acad, Pharmacia Biotech, Antigen Measures, Equipment End-over-end, Reagents Buffer, Wash the Sepharose
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