Antibody Phage Display (Methods in Molecular Biology) [Hardcover]

Philippa M. O'Brien (Editor), Robert Aitken (Editor)

Book Description

ISBN-10: 0896039064 | ISBN-13: 978-0896039063 | Publication Date: December 15, 2001 | Edition: 1st

This comprehensive collection of established antibody phage display protocols features authoritative guidance that will enable the nonspecialist successfully to carry them out. Coverage spans the construction of antibody libraries, the selection of antibody clones with the desired properties, and their modification, expression, and purification. Comprehensive and highly practical, Antibody Phage Display: Methods and Protocols provides biochemists, molecular biologists, and immunologists with a gold-standard reference guide to the successful isolation, modification, and expression of recombinant antibodies using today's powerful phage display technology.

Editorial Reviews

Review

"...this book lives up to its promises. It starts with an excellent introduction by Hennie Hoogenboom, reprinted from his Immunotechnology article, which sets the scene and provides an overview of the field for new readers...Members of the laboratory that used the book for reference found it very clear and all were keen that the review was completed as quickly as possible so that the book was returned to the laboratory where they could make use of it! I think this in itself supports the fact that the book is a useful volume." - Immunology News

"As with other volumes in this popular series, each protocol is discussed in detail, including an introduction, materials, methods notes, and references. It is a good purchase for research libraries where monoclonal antibodies are studied." E-Streams

From the Back Cover

Since its introduction almost 20 years ago, phage display technology has revolutionized approaches to the analysis of biomedical problems, quickly impacting the fields of  immunology, cell biology, biotechnology, pharmacology, and drug discovery. In Antibody Phage Display: Methods and Protocols, Second Edition, expert researchers explore the latest in this cutting-edge technology, providing an invaluable resource that will guide readers in the design and execution of experiments based around antibody phage display. Chapters present a wide range of methods of isolating recombinant antibodies from phage display libraries, examine how the targets recognized by antibodies of interest can be identified, discuss the identification and exploitation of antibodies that can enter cells and bind to cytosolic targets, and include novel approaches to the expression of recombinant antibodies. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Detailed and innovative, Antibody Phage Display: Methods and Protocols, Second Edition is a critical handbook on phage display technology which is certain to stimulate the reader’s imagination as much as it will guide future practice in the laboratory. --This text refers to an alternate Hardcover edition.

Product Details

• Hardcover: 416 pages
• Publisher: Humana Press; 1st edition (December 15, 2001)
• Language: English
• ISBN-10: 0896039064
• ISBN-13: 978-0896039063
• Product Dimensions: 9 x 6.1 x 1.4 inches
• Shipping Weight: 1.9 pounds
• Amazon Best Sellers Rank: #7,128,767 in Books (See Top 100 in Books)