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Automated DNA Sequencing and Analysis
 
 
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Automated DNA Sequencing and Analysis [Hardcover]

Mark D. Adams (Editor), Chris Fields (Editor), J. Craig Venter (Editor)

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Book Description

0127170103 978-0127170107 July 13, 1994 1
A timely book for DNA researchers, Automated DNA Sequencing and Analysis reviews and assesses the state of the art of automated DNA sequence analysis-from the construction of clone libraries to the developmentof laboratory and community databases. It presents the methodologies and strategies of automated DNA sequence analysis in a way that allows them to be compared and contrasted. By taking a broad view of the process of automated sequence analysis, the present volume bridges the gap between the protocols supplied with instrument and reaction kits and the finalized data presented in the research literature. It will be an invaluable aid to both small laboratories that are interested in taking maximum advantageof automated sequence resources and to groups pursuing large-scale cDNA and genomic sequencing projects.

* The field of automation in DAN sequencing and analysis is rapidly moving. Hovever, as the technology becomes commonplace, those applying the techniques involved to their research fields need a text which both expands on the protocols supplied by manufacturers with their instruments and explains how to utilise the data produced. This book fulfils those needs, reviews the history of the art and provides pointers to future development.

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From the Back Cover

This book reviews and assesses the state of the art of automated DNA sequence analysis, from the construction of clone libraries to the development of laboratory and community databases. It presents the methodologies and strategies of automated DNA sequence analysis in a way that allows them to be compared and contrasted. By taking a broad view of the process of automated sequence analysis, the present volume bridges the gap between the protocols supplied with instrument and reaction kits and the finalized data presented in the research literature. It is targeted both at small laboratories that are interested in taking maximum advantage of automated sequencing resources and groups pursuing large-scale cDNA and genomic sequencing projects.

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Inside This Book (learn more)
First Sentence:
The GenBank genetic sequence database, which contained only 15 million nucleotides in 1987, has nearly doubled its size in each of the subsequent 5 years. Read the first page
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
final consensus sequence, final contig, magnetic module, contig editor, nested deletion sets, contextual arrays, contig merge, reverse sequencing, trace editor, frameshift errors, binary coding method, termination mixture, cosmid sequence, oligomer yields, candidate exons, consistent coverings, phage supernatant, abundant clones, cycle sequencing reactions, oligomer separations, thermal ramp, overlap strength, sequencing buffer, chimeric clones, sequencing templates
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Nucleic Acids Res, Nature Genet, Methods Enzymol, Cold Spring Harbor, Applied Biosystems, New York, Academic Press, San Diego, Department of Energy, Molecular Cloning Techniques, Computer Society Press, Technical Report, Hawaii International Conference, Department of Computer Science, Boehringer Mannheim, Los Alamitos, Los Alamos National Laboratory, System Sciences, University of Arizona, Gene Clean, Laboratory Manual, Lawrence Berkeley Laboratory, Human Genome Center, Palo Alto, Practical Approach
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