For both volumes: Expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1 presents readily reproducible techniques for library construction, physical mapping, and sequencing.. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies.
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The ability to transfer and maintain DNA both within and between species is an essential skill in biotechnology and medicine. In Bacterial Artificial Chromosomes, expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1: Library Construction, Physical Mapping, and Sequencing presents readily reproducible techniques for library construction, physical mapping, and sequencing. The laboratory protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Comprehensive and cutting-edge, the two volumes of Bacterial Artificial Chromosomes provide a superlative collection of highly productive protocols that will prove useful to many bioscientists, including genome sequencers, geneticists, molecular biologists, and biochemists studying the structure and function of specific genomes.
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Inside This Book
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First Sentence:
DNA cloning, especially large DNA cloning, is the first step in contemporary complex genome analysis. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
transposon reaction, new copy plates, equilibrate the unit, alkaline purification, contig file, retrofitting vector, graphical tool for sequence finishing, align files, preparative slot, primer pool, blue loading dye, fragment end repair, seq file, phred scores, custom primers, false join, finishing reads, nonrecombinant clones, partial digestion condition, kanamycin stock solution, deletion clones, sequencing mix, tryptone peptone, bacterial artificial chromosome library, enzyme dilution buffer Key Phrases - Capitalized Phrases (CAPs): (learn more)
New England Biolabs, Life Technologies, Genome Res, Nucleic Acids Res, Humana Press Inc, Sequencing Edited, Applied Biosystems, Optimized Multiplex, Amersham Pharmacia Biotech, Cold Spring Harbor, Luria Bertani, Low Range, Oak Ridge, Alpha Innotech, Human Genome Project, Perkin Elmer, Dye Terminator Mix, Materials All, Molecular Cloning, New York, Sanger Centre, Stanford Human Genome Center, Foster City, Gel Extraction Kit, National Cancer Institute New!
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Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
DNA cloning, especially large DNA cloning, is the first step in contemporary complex genome analysis. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
transposon reaction, new copy plates, equilibrate the unit, alkaline purification, contig file, retrofitting vector, graphical tool for sequence finishing, align files, preparative slot, primer pool, blue loading dye, fragment end repair, seq file, phred scores, custom primers, false join, finishing reads, nonrecombinant clones, partial digestion condition, kanamycin stock solution, deletion clones, sequencing mix, tryptone peptone, bacterial artificial chromosome library, enzyme dilution buffer Key Phrases - Capitalized Phrases (CAPs): (learn more)
New England Biolabs, Life Technologies, Genome Res, Nucleic Acids Res, Humana Press Inc, Sequencing Edited, Applied Biosystems, Optimized Multiplex, Amersham Pharmacia Biotech, Cold Spring Harbor, Luria Bertani, Low Range, Oak Ridge, Alpha Innotech, Human Genome Project, Perkin Elmer, Dye Terminator Mix, Materials All, Molecular Cloning, New York, Sanger Centre, Stanford Human Genome Center, Foster City, Gel Extraction Kit, National Cancer Institute New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
This book cites 16
books:
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- Molecular Cloning: A Laboratory Manual, Third Edition (3 volume set) by Joe Sambrook on page 68, and page 210
- Nucleic Acids Sequencing by Howe on page 186
- Parasite Genomics Protocols (Methods in Molecular Biology) by Sara E. Melville in Front Matter
- Nucleic Acids Sequencing: A Practical Approach (Practical Approach Series) by C. J. Howe on page 186
- Medical Research (Finding Out About) by Martin Hughes in Front Matter
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