Basic Methods in Microscopy: Protocols and Concepts from Cells: A Laboratory Manual [Paperback]

David L. Spector (Author), Robert D. Goldman (Author)

Book Description

Publication Date: September 30, 2005 | ISBN-10: 0879697512 | ISBN-13: 978-0879697518 | Edition: 1st

Imaging has become a vital tool for researchers in all aspects of biology. Recent advances in microscope technology, labeling techniques and gene and protein manipulation methods have led to breakthroughs in our understanding of biological processes. In order to take advantage of these techniques, biologists need to understand the fundamental techniques of microscopy. The methods found here, drawn from the popular laboratory standard manuals Cells: A Laboratory Manual and Live Cell Imaging provide a solid course in the basics of using the microscope in a biology laboratory. Basic Methods in Microscopy provides an essential guide to light microscopy, fluorescence microscopy, confocal microscopy, multiphoton microscopy and electron microscopy, preparation of tissues and cells, labeling of specimens and analysis of cellular events. This manual is an important tool for any biology researcher employing imaging as a research method. Related Titles from the Publisher Live Cell Imaging: A Laboratory Manual Cells: A Laboratory Manual Molecular Cloning: A Laboratory Manual Imaging in Neuroscience and Development: A Laboratory Manual Lab Math Lab Ref, Volume 2: A Handbook of Recipes, Reagents, and Other Reference Tools for Use at the Bench Lab Dynamics: Management Skills for Scientists At the Bench At the Helm

Editorial Reviews

Review

In summary, this book is a useful reference for a teaching laboratory or a microscopy facility with very general interests in a variety of microscopy techniques primarily for fixed tissues. Microbiology Today Any research laboratory that uses microscopy would benefit immensely by having this volume on hand. The components, from underlying optical principles and theories behind the most basic light microscope to the highly advanced transmission electron microscope, are clearly described in the text, figures, and legends. A variety of techniques in fluorescence microscopy are presented, including protocols for the preparation of specimens, preparation of slides and cover slips, and proper care, maintenance, and cleaning of optical equipment. A handy troubleshooting section provides guidance on how to correct imaging problems. The Quarterly Review of Biology --Cold Spring Harbor

Product Details

• Paperback: 375 pages
• Publisher: Cold Spring Harbor Laboratory Press; 1st edition (September 30, 2005)
• Language: English
• ISBN-10: 0879697512
• ISBN-13: 978-0879697518
• Product Dimensions: 11 x 8.3 x 0.7 inches
• Shipping Weight: 2.4 pounds (View shipping rates and policies)
• Average Customer Review: 4.0 out of 5 stars  See all reviews (1 customer review)
• Amazon Best Sellers Rank: #1,259,045 in Books (See Top 100 in Books)

Customer Reviews

Most Helpful Customer Reviews

6 of 7 people found the following review helpful:

4.0 out of 5 stars A book with useful protocols, March 14, 2008

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Lingyan Wang - See all my reviews
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This review is from: Basic Methods in Microscopy: Protocols and Concepts from Cells: A Laboratory Manual (Paperback)

This is a book covering the principles of all kinds of microscopes with structure figures. There many good color photos and graphs. The protocols of tissue preparations are very useful, but maybe duplicate in other books. If you just want to know how to use the microscopes instead of understanding them, it should be a good book for you. It is more like a lab manual collection.

The problem of this book is that it distracts a little bit from microscopes to cell culture and immunohistochemistry.