Yes, but just barely and depends on how you configure the inside. My Canon 60D with battery grip just barely fits the height of the grip and body. It causes the flap to bulge slightly with my current configuration which allows me to store a 580EXii flash, 50mm, 27-85 mounted on body, and a 55-250mm all in the main compartment with room to spare in the top and front pockets
It's a nice bag but not deep enough for my Canon 7D with battery grip. The bag was noticeably distorted when I zipped up the flap which would have made it difficult to get to anything stored along the sides of the bag and I returned the bag to Amazon and bought a larger(and more expensive) Lowepro Flipside 300 backpack.