C. elegans: A Practical Approach [Hardcover]

Ian A. Hope (Editor)

Book Description

ISBN-10: 0199637393 | ISBN-13: 978-0199637393 | Publication Date: March 9, 2000 | Edition: 1

Caenorhabditis Elegans has been a popular model organism for biological research for over thirty years and has been used to investigate many aspects of animal development, for example apoptosis, the Hox genes, signal transduction pathways, and the development of the nervous system. It has recently taken on new importance with the publication of its entire genome sequence in 1998. This book contains all the basic information on C. elegans required to use it: it's natural history, anatomy, life cycle, development, and evolution; information on how to obtain, grow, and maintain C. elegans for use as a model system; the genome project and how to use genome sequence information by searching the database for homologues using different search methods and then how to analyse the search data; the essential practical details of transformation and common uses for the technique; and how to analyse phenotype by microscopy, including Normaski, fluorescence, 4-dimensional, and electron microscopy. Reverse genetics and strategies for gene inactivation that are known to work in C elegans are also outlined, such as epigenetic inactivation and mutational germ line inactivation. Techniques for studying the neurobiology of C. elegans are given as well as information on conventional genetics and details of the standard procedures for selfing and crossing; mutagenesis and mutant screening; characterization of mutants; gene mapping; temperature-shift experiments and mosaic analysis. All in all, this is the ideal book for those looking to know anything from applicability to the biology of C. elegans.

Editorial Reviews

Review

"Provides information for researchers working with Caenorhabditis elegans, giving basic background on the organism's natural history, anatomy, life cycle, and evolution, and then detailing methods for using C. elegans in research. Tells how to obtain and grow C. elegans, and discusses the C. elegans genome project, transformation of C. elegans, reverse genetic techniques, analysis of phenotypes by various methods, and approaches for studying gene expression. Describes procedures for producing biochemically useful preparations, and details conventional genetics procedures for selfing and crossing, mutagenesis and mutant screening, gene mapping, and mosaic analysis. Hope teaches biology at the University of Leeds."--SciTech Book News

About the Author

Ian A. Hope is at University of Leeds.

Product Details

• Hardcover: 304 pages
• Publisher: Oxford University Press, USA; 1 edition (March 9, 2000)
• Language: English
• ISBN-10: 0199637393
• ISBN-13: 978-0199637393
• Product Dimensions: 9.3 x 5.8 x 0.9 inches
• Shipping Weight: 1.2 pounds (View shipping rates and policies)
• Average Customer Review: 4.0 out of 5 stars  See all reviews (1 customer review)
• Amazon Best Sellers Rank: #1,190,857 in Books (See Top 100 in Books)

Customer Reviews

Most Helpful Customer Reviews

4.0 out of 5 stars C. elegans for begginers and experts, October 26, 2010

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Marcela - See all my reviews

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This review is from: C. elegans: A Practical Approach (Paperback)

The book is exactly what you need if you are starting to deal with C. elegans. It contains both basic and complex protocols and for a wide variety of worm organs.
In addition there is an appendix with useful web links to C. elegans community.