Capillary Electrophoresis of Proteins and Peptides (Methods in Molecular Biology (Clifton, N.J.), V. 276.) (Methods in Molecular Biology) [Hardcover]

Mark A. Strege (Editor), Avinash L. Lagu (Editor)

Book Description

ISBN-10: 1588290174 | ISBN-13: 978-1588290175 | Publication Date: June 3, 2004 | Edition: 1

Expert academic and pharmaceutical researchers describe their best capillary electrophoresis techniques for protein and peptide analysis. The authors present cutting-edge techniques for both capillary coatings and analytic detection via laser-induced fluorescence, for the development and commercialization of biopharmaceuticals, and for affinity capillary electrophoresis in the evaluation of protein binding, including the use of protein charge ladders. Areas of special interest covered include combining CE and capillary isoelectric focusing with electrospray mass spectrometry detection to perform proteomic studies and applications in the field of protein-ligand binding.

Editorial Reviews

From the Back Cover

Capillary electrophoresis (CE) is one of the premier methods for the rapid analysis of pharmaceutical proteins and peptides, and thus now even more important with the sequencing of the human genome and the subsequent advent of proteomics. In Capillary Electrophoresis of Proteins and Peptides, leading academic scientists and expert laboratorians working in the pharmaceutical industry describe their best CE techniques in step-by-step detail for easy reproduction. The authors present cutting-edge techniques for both capillary coatings and analytical detection via laser-induced fluorescence, for the development and commercialization of biopharmaceuticals, and for affinity capillary electrophoresis in the evaluation of protein binding, including the use of protein charge ladders. Additional chapters discuss CE and capillary isoelectric focusing, combined with electrospray mass spectrometry detection, for performing proteomic studies. Areas of special interest covered include proteomic applications, as well as applications in the field of protein-ligand binding. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Capillary Electrophoresis of Proteins and Peptides will prove essential to both new and experienced investigators carrying out significant protein and peptide studies in biotechnology, drug discovery, and pharmaceutical research and development.

Product Details

• Hardcover: 344 pages
• Publisher: Humana Press; 1 edition (June 3, 2004)
• Language: English
• ISBN-10: 1588290174
• ISBN-13: 978-1588290175
• Product Dimensions: 9.4 x 6.5 x 1.2 inches
• Shipping Weight: 1.7 pounds (View shipping rates and policies)
• Average Customer Review: 5.0 out of 5 stars  See all reviews (1 customer review)
• Amazon Best Sellers Rank: #1,860,277 in Books (See Top 100 in Books)

Customer Reviews

Most Helpful Customer Reviews

5.0 out of 5 stars An excellent volume for browsing or reference, February 17, 2010

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David Zalewski "After-hours science reader" (The big pharm in Indiana) - See all my reviews
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This review is from: Capillary Electrophoresis of Proteins and Peptides (Methods in Molecular Biology (Clifton, N.J.), V. 276.) (Methods in Molecular Biology) (Hardcover)

Obviously most of us will be introduced to this subject through a vendor and an expensive "turn-key" system, but the editors and contributors to this volume did an excellent job of summarizing a variety of approaches to the theory and applications of CE. This field is obviously still ahead of it's time, mostly academic, and a great topic for graduate research. Certainly many applications have been replaced with more sophisticated techniques like mass spectrometry, but for those with even minimal time, it's great to consider separation inside of a very small tube by several forces simultaneously, very quickly and with extremely high resolution. Also, the physical and chemical environment lend themselves to experimental designs involving many kinds of interactions on a chemical level (such as complexation with ligands, even including colorimetric applications which might not be as simple with ordinary liquid chromatography) over an extremely fast time scale. This may be a nice book that is only appreciated after another decade passes and industry has actually hired enough innovative scientists that are daring enough to try some experiments on their own time and force some necessary cultural changes.