Celiac Disease: Methods and Protocols (Methods in Molecular Medicine) [Hardcover]

Michael N. Marsh (Editor)

Book Description

ISBN-10: 0896036502 | ISBN-13: 978-0896036505 | Publication Date: April 1, 2000 | Edition: 1

Professor Michael N. Marsh, a recognized world authority on this condition, together with a team of other expert laboratory/clinical investigators from around the world, present a collection of the state-of-the-art techniques for studying the biology and immunopathology of celiac disease. Both the novice and experienced researcher will find in this collection detailed step-by-step methods for cloning lymphocytes, creating gene/peptide libraries, and performing genotyping, linkage, and positional cloning. Also included are techniques for determining the peptide structure of HLA-bound material (tandem mass spectroscopy), computerized morphometry, in situ hybridization, organ culture, and monoclonal AB assays. Comprehensive and cutting-edge, Celiac Disease: Methods and Protocols provides all experimental and clinical investigators with the essential core of readily reproducible methods necessary for successful work on celiac disease today.

Editorial Reviews

Review

"Of interest to gastroenterologists, pathologists and those working in research on celiac disease, a disease linked by a so far unknown mechanism to type 1 diabetes." -Journal of Pediatric Endocrinology & Metabolism

From the Back Cover

With the recent development of powerful bioanalytical techniques, research on gluten sensitivity has entered an exciting new phase. In Celiac Disease: Methods and Protocols, Professor Michael N. Marsh, a recognized world authority on this condition, together with a team of other expert laboratory/clinical investigators from around the world present a collection of these state-of-the-art techniques for studying the biology and immunopathology of celiac disease. Both novice and experienced researcher will find in this collection detailed step-by-step methods for cloning lymphocytes, creating gene/peptide libraries, and performing genotyping, linkage, and positional cloning. Also included are techniques for determining the peptide structure of HLA-bound material (tandem mass spectroscopy), computerized morphometry, in situ hybridization, organ culture, and monoclonal AB assays. Comprehensive and cutting-edge, Celiac Disease: Methods and Protocols provides all experimental and clinical investigators with the essential core of readily reproducible methods necessary for successful work on celiac disease today.

Product Details

• Hardcover: 304 pages
• Publisher: Springer; 1 edition (April 1, 2000)
• Language: English
• ISBN-10: 0896036502
• ISBN-13: 978-0896036505
• Product Dimensions: 9.3 x 6.4 x 1 inches
• Shipping Weight: 1.5 pounds (View shipping rates and policies)
• Amazon Best Sellers Rank: #3,376,945 in Books (See Top 100 in Books)