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Cloning, Gene Expression, and Protein Purification: Experimental Procedures and Process Rationale Paperback – March 1, 2001

ISBN-13: 978-0195132946 ISBN-10: 0195132947 Edition: 1st

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Product Details

  • Paperback: 448 pages
  • Publisher: Oxford University Press; 1 edition (March 1, 2001)
  • Language: English
  • ISBN-10: 0195132947
  • ISBN-13: 978-0195132946
  • Product Dimensions: 0.8 x 8.4 x 10.8 inches
  • Shipping Weight: 2.3 pounds (View shipping rates and policies)
  • Average Customer Review: 5.0 out of 5 stars  See all reviews (1 customer review)
  • Amazon Best Sellers Rank: #1,843,228 in Books (See Top 100 in Books)

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About the Author


Charles Hardin is an Associate Professor of Biochemistry at North Carolina State University. Jennifer Pinczes recently completed her Master's of Science in biochemistry at North Carolina State University. Andrew Riell is a computer specialist and consultant with NetAspects, Inc. William Miller is William Neals Reynolds Professor of Biochemistry at North Carolina State University. David Presutti is an Adjunct Visiting Professor of Biochemistry at North Carolina State University. Dominique Robertson is an Associate Professor of Botany at North Carolina State University.

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I actually have Presutti as my instructor for this course and man I'll tell you, he's quite the character. Dave tends to skip some parts of the procedures and has his own organization of how he wants us to go through it. Sometimes he'll even create side procedures to "update" parts of the original manual's procedures since he's using a newer or cheaper chemical or material for that part.

When looking through it this manual is in serious need of an update when compared to other lab manuals but overall it's still an impressive procedural manual. It provides very straight forward lab procedures that along with instructor background information and some individual external learning, should educate any opportunistic students dreaming of becoming an awesome future researcher, such as Dave. Personally going through many of these experiments it's obvious that results won't be as expected but as Dave always reminds us, you wouldn't learn anything if it was perfect. So gear up with this awesome lab manual and tackle that lab class down for an A.
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