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5.0 out of 5 stars
Why so many people say that two photons are better than one?, May 16, 2006
This review is from: Confocal and Two-Photon Microscopy: Foundations, Applications and Advances (Hardcover)
Reviewing Confocal and Two-Photon Microscopy: Foundations, Applications, and Advances, a difficult question to answer could be: is this text a "book" or a "manual"? The best answer is probably "both". The advantages of both approaches to the subject are present in the text: it provides a clear, neat overview of the theoretical principles and basic foundations of modern laser-scanning microscopy and a large variety of advanced applications including detailed technical descriptions and practical hints.
This book takes the reader through very complex phenomena and the basic concepts of confocal and two-photon microscopy, fluorescence imaging, digital image processing and image-restoration methods are clearly explained. In addition, application chapters interestingly cover not only the great advantages of the quantitative laser-scanning microscopy in biology but also the practical complexity and the limitations of the technique.
In the end, a remarkable text of "theory and practice" of the most relevant present light microscopy techniques.
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