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Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications 6th Edition

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ISBN-13: 978-0470528129
ISBN-10: 0470528125
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Editorial Reviews

Review

"The book's sixth incarnation includes new sections on organotypic culture and different cell and stem cell types". (Veterinary Times, 11 April 2011)  

From the Back Cover

The most complete resource on the techniques, equipment, principles, and practices of animal cell culture

Since publication of the previous edition of this benchmark text, numerous types of equipment and materials have changed and new specialized techniques have been developed, particularly in the area of stem cell research and tissue engineering. These and other developments have been incorporated into this rigorously revised and expanded Sixth Edition of Culture of Animal Cells, the leading text in the basic methodology of cell culture.

This authoritative volume offers a complete background related to growth of animal cells in culture. It contains many specialized protocols and advice on how to apply some of the basic protocols to teaching cell culture. Beginning with laboratory design, safety, validation, and bioethics, then continuing with preparation of media, primary culture, and cell lines through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the unparalleled coverage includes:

  • An all-new section addressing the additional caution required once a primary culture is converted to a cell line

  • Differentiation in stem cells and reprogramming of adult cells to form stem cells

  • A new section and protocol on cryopreservation by vitrification

  • Separate sections dealing with epithelial cells, mesenchymal cells, neurectodermal cells, and hematopoietic cells, a new chapter on germs cells/stemcells/amniocytes and non-mammalian/avain cells (other vertebrates and invertebrates)

  • A new section on organotypic culture

  • Increased emphasis on contamination and misidentification (including cross-contamination) with revised protocols for detection and elimination

  • A problem-solving chapter, glossary of terms, advice on sourcing materials, and list of suppliers

This landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells and is indispensable for those in the field, including clinical and biopharmaceutical researchers and scientists, graduate students, laboratory managers in cell and molecular biology labs and genetics labs, trainees, and technicians.

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Product Details

  • Hardcover: 796 pages
  • Publisher: Wiley-Blackwell; 6 edition (September 17, 2010)
  • Language: English
  • ISBN-10: 0470528125
  • ISBN-13: 978-0470528129
  • Product Dimensions: 8.8 x 1.8 x 11.3 inches
  • Shipping Weight: 4.6 pounds (View shipping rates and policies)
  • Average Customer Review: 4.0 out of 5 stars  See all reviews (10 customer reviews)
  • Amazon Best Sellers Rank: #276,197 in Books (See Top 100 in Books)

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Customer Reviews

Most Helpful Customer Reviews

5 of 5 people found the following review helpful By Fabio Santos on April 12, 2011
Format: Hardcover Verified Purchase
The book is very good. Information very well detailed, including experimental protocols. Essential book for anyone who works or wants to work with cultured animal cells.
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1 of 1 people found the following review helpful By Susan E Brown on June 26, 2012
Format: Hardcover Verified Purchase
This book absolutely contains a wealth of knowledge. However, it is written with the assumption that the reader is already very familiar with many tissue culture and bio-lab terms and concepts. Terms are used without being defined, and many of the technical terms are not located in the glossary. The book is written more in the format of a formal scientific paper. Information is provided, but not necessarily explained. I have no doubt that this book would be a good reference for a laboratory professional who wants to transition to cell culture from a similar field. If you are a student like me, chances are good your current education is not sufficient to appreciate the full value of this text, but instead find it frusturating and painful to read.
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1 of 1 people found the following review helpful By Mike Noel on July 11, 2013
Format: Hardcover Verified Purchase
This text gives a thorough account of cell culturing as well as the protocols and concepts of maintaining cell lines in a laboratory. The text gives insights to help troubleshoot any difficulties encountered. It was worth the purchase. I recommend this textbook especially anyone who is considering starting the own lab that includes cell culture.
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Format: Hardcover Verified Purchase
I will highly recommend this book, if you are looking for something helpful in cell culture area. I set up my cell culture lab by the guidance of this book which is comperehensive and precise. You can have it as your friend and whenever you encounter a problem in cell culture refer to it. I am so satisfied of having this book by my side.
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Format: Hardcover Verified Purchase
The information was laid out very precisely so that the text was easy to read. It did seem a bit dated, though not inaccurate. I would have liked if the pictures inside were colored rather than gray scale, since it is such a fascinating subject.
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Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications
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