Culture of Animal Cells and over one million other books are available for Amazon Kindle. Learn more

Sorry, this item is not available in
Image not available for
Color:
Image not available

To view this video download Flash Player

 


or
Sign in to turn on 1-Click ordering
Sell Us Your Item
For a $32.57 Gift Card
Trade in
More Buying Choices
Have one to sell? Sell yours here
Start reading Culture of Animal Cells on your Kindle in under a minute.

Don't have a Kindle? Get your Kindle here, or download a FREE Kindle Reading App.

Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications [Hardcover]

R. Ian Freshney
4.1 out of 5 stars  See all reviews (8 customer reviews)

Buy New
$84.93 & FREE Shipping. Details
Rent
$22.00
Only 10 left in stock (more on the way).
Ships from and sold by Amazon.com. Gift-wrap available.
In Stock.
Want it tomorrow, July 15? Choose One-Day Shipping at checkout. Details
Free Two-Day Shipping for College Students with Amazon Student

Formats

Amazon Price New from Used from
Kindle Edition $79.96  
Hardcover $84.93  

Book Description

September 17, 2010 0470528125 978-0470528129 6
This is the most complete resource on the techniques, equipment, principles, and practices of animal cell culture.

Since publication of the previous edition of this benchmark text, numerous types of equipment and materials have changed and new specialized techniques have been developed, particularly in the area of stem cell research and tissue engineering. These and other developments have been incorporated into this rigorously revised and expanded sixth edition of Culture of Animal Cells, the leading text in the basic methodology of cell culture.

This authoritative volume offers a complete background related to growth of animal cells in culture. It contains many specialized protocols and advice on how to apply some of the basic protocols to teaching cell culture. Beginning with laboratory design, safety, validation, and bioethics, then continuing with preparation of media, primary culture, and cell lines through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the unparalleled coverage includes:

  • An all-new section addressing the additional caution required once a primary culture is converted to a cell line

  • Differentiation in stem cells and reprogramming of adult cells to form stem cells

  • A new section and protocol on cryopreservation by vitrification

  • Separate sections dealing with epithelial cells, mesenchymal cells, neurectodermal cells, and hematopoietic cells, a new chapter on germs cells/stemcells/amniocytes

  • A new section on organotypic culture

  • Increased emphasis on contamination and misidentification (including cross-contamination) with revised protocols for detection and elimination

  • A problem-solving chapter, glossary of terms, advice on sourcing materials, and list of suppliers

This landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells and is indispensable for those in the field, including clinical and biopharmaceutical researchers and scientists, graduate students, laboratory managers in cell and molecular biology labs and genetics labs, trainees, and technicians.

Q&A with Author R. Ian Freshney, PhD.
Author R. Ian Freshney, PhD.
R. Ian Freshney, PhD, is an honorary Senior Research Fellow in the Centre for Oncology and Applied Pharmacology at the University of Glasgow. He is a world-renowned expert on cell culture technique and has authored and edited numerous successful books, including the hugely popular Culture of Animal Cells (now in its 6th Edition), and Culture of Human Stem Cells.

Culture of Animal Cells: A Manual of Basic Technique and Specialized Application, 6th Ed has been very well received by the scientific community, as have many of your other titles. What do you think sets your books apart and makes them so successful compared to other titles in the field?
I would say there are three major components: (1) The books are designed as practical guides with detailed single-step instructions which should be sufficient without further recourse to the literature. (2) The preparation and sources of materials are explained in detail. (3) Most of the basic protocols are illustrated with easy-to-follow diagrams.

What challenges did you face while putting together Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, 6th Edition?
I struggled with the need to update but at the same time preserve basic procedures which have not changed markedly from the previous edition. This, of course, led to the dilemma of deciding what to retain and what to leave out. I also thought long and hard about what specialized protocols to add without compromising the basic technique role of the book.

What do you find rewarding about authoring and editing books in the sciences?
It forces me to keep up-to-date with developments in cell culture, and in turn helps me to be a better teacher of cell culture. I also get satisfaction from converting what are sometimes complex instructions into one simple straightforward procedure.

What inspired you to write your first book?
I was invited to prepare a textbook on cell culture for a college course. Previously, I had used John Paul’s book, Cell and Tissue Culture, and knew it needed to be updated.

What advice would you offer to scientists writing or editing their first book?
- Determine the precise niche that you wish to fill.
- Plan the structure carefully beforehand but do not be afraid to deviate, within reason, to meet new demands.
- Ensure the text is well illustrated with tables and diagrams.
- Make sure the book is properly cross-referenced.
- Prepare the index yourself. Even if it is to be professionally indexed you will find terms that the indexer does not.

How would you compare the experiences of writing a book and writing a journal article?
Both require a clear concise style, but the author has more freedom to express ideas and opinions when writing a book. Another major difference is that a scientific paper must address a specific topic and provide proof to substantiate the conclusions that are drawn, while a book draws on the author’s and other scientists’ experience to provide a more general review and specific guidelines or instructions.

Do you find that the role of books in the scientific research community has changed over the years? Are they valued more or less today than they were 10-20 years ago?
Rapid changes in technology mean that some books will become outdated without regular updates, which are not always feasible in printed copy. Yet, while there is an increasing tendency to provide instructions and protocols online, many people will still find reassurance from using a textbook with an established reputation.

How do you envision the evolution of science writing over the next 5-10 years?
Certain journals will still tend to be regarded as more reliable than others, as indicated by their citation indices. However, the number of free public access journals will probably increase, presumably with payment to submit articles. There will be a continuing need for editorial control of journal content due to the proliferation of unedited, non-reviewed material appearing online.

What do you feel has been the most significant scientific discovery that has been made during your lifetime?
I can really only speak for biology where the elaboration of the genetic code is probably the most significant development. The ability to regulate gene expression and the resultant plasticity of the cell phenotype will create major opportunities in cell culture and its relevance to tissue in vivo.

What are you reading right now?
This questionnaire! Otherwise, mostly novels and current journals for which I have alerts.


Frequently Bought Together

Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications + The Immortal Life of Henrietta Lacks
Price for both: $93.97

Buy the selected items together
  • The Immortal Life of Henrietta Lacks $9.04

Customers Who Bought This Item Also Bought


Editorial Reviews

Review

"The book's sixth incarnation includes new sections on organotypic culture and different cell and stem cell types". (Veterinary Times, 11 April 2011)  

From the Back Cover

The most complete resource on the techniques, equipment, principles, and practices of animal cell culture

Since publication of the previous edition of this benchmark text, numerous types of equipment and materials have changed and new specialized techniques have been developed, particularly in the area of stem cell research and tissue engineering. These and other developments have been incorporated into this rigorously revised and expanded Sixth Edition of Culture of Animal Cells, the leading text in the basic methodology of cell culture.

This authoritative volume offers a complete background related to growth of animal cells in culture. It contains many specialized protocols and advice on how to apply some of the basic protocols to teaching cell culture. Beginning with laboratory design, safety, validation, and bioethics, then continuing with preparation of media, primary culture, and cell lines through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the unparalleled coverage includes:

  • An all-new section addressing the additional caution required once a primary culture is converted to a cell line

  • Differentiation in stem cells and reprogramming of adult cells to form stem cells

  • A new section and protocol on cryopreservation by vitrification

  • Separate sections dealing with epithelial cells, mesenchymal cells, neurectodermal cells, and hematopoietic cells, a new chapter on germs cells/stemcells/amniocytes and non-mammalian/avain cells (other vertebrates and invertebrates)

  • A new section on organotypic culture

  • Increased emphasis on contamination and misidentification (including cross-contamination) with revised protocols for detection and elimination

  • A problem-solving chapter, glossary of terms, advice on sourcing materials, and list of suppliers

This landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells and is indispensable for those in the field, including clinical and biopharmaceutical researchers and scientists, graduate students, laboratory managers in cell and molecular biology labs and genetics labs, trainees, and technicians.


Product Details

  • Hardcover: 796 pages
  • Publisher: Wiley-Blackwell; 6 edition (September 17, 2010)
  • Language: English
  • ISBN-10: 0470528125
  • ISBN-13: 978-0470528129
  • Product Dimensions: 11 x 8.7 x 1.6 inches
  • Shipping Weight: 4.6 pounds (View shipping rates and policies)
  • Average Customer Review: 4.1 out of 5 stars  See all reviews (8 customer reviews)
  • Amazon Best Sellers Rank: #356,008 in Books (See Top 100 in Books)

More About the Author

Discover books, learn about writers, read author blogs, and more.

Customer Reviews

4.1 out of 5 stars
(8)
4.1 out of 5 stars
Share your thoughts with other customers
Most Helpful Customer Reviews
5 of 5 people found the following review helpful
5.0 out of 5 stars Great Book April 12, 2011
Format:Hardcover|Verified Purchase
The book is very good. Information very well detailed, including experimental protocols. Essential book for anyone who works or wants to work with cultured animal cells.
Comment | 
Was this review helpful to you?
1 of 1 people found the following review helpful
4.0 out of 5 stars text review July 11, 2013
Format:Hardcover|Verified Purchase
This text gives a thorough account of cell culturing as well as the protocols and concepts of maintaining cell lines in a laboratory. The text gives insights to help troubleshoot any difficulties encountered. It was worth the purchase. I recommend this textbook especially anyone who is considering starting the own lab that includes cell culture.
Comment | 
Was this review helpful to you?
1 of 1 people found the following review helpful
2.0 out of 5 stars Not meant for students June 26, 2012
Format:Hardcover|Verified Purchase
This book absolutely contains a wealth of knowledge. However, it is written with the assumption that the reader is already very familiar with many tissue culture and bio-lab terms and concepts. Terms are used without being defined, and many of the technical terms are not located in the glossary. The book is written more in the format of a formal scientific paper. Information is provided, but not necessarily explained. I have no doubt that this book would be a good reference for a laboratory professional who wants to transition to cell culture from a similar field. If you are a student like me, chances are good your current education is not sufficient to appreciate the full value of this text, but instead find it frusturating and painful to read.
Comment | 
Was this review helpful to you?
5.0 out of 5 stars Five Stars July 10, 2014
Format:Hardcover|Verified Purchase
Useful books for my course and for lab operation
Comment | 
Was this review helpful to you?

What Other Items Do Customers Buy After Viewing This Item?


Forums

There are no discussions about this product yet.
Be the first to discuss this product with the community.
Start a new discussion
Topic:
First post:
Prompts for sign-in
 



Look for Similar Items by Category