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Culture of Human Stem Cells (Culture of Specialized Cells)
 
 
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Culture of Human Stem Cells (Culture of Specialized Cells) [Hardcover]

R. Ian Freshney (Author), Glyn N. Stacey (Author), Jonathan M. Auerbach (Author)

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Book Description

June 22, 2007 0470052465 978-0470052464 1
This book collects the most effective and cutting-edge methods and protocols for deriving and culturing human embryonic and adult stem cells—in one handy resource.

This groundbreaking book follows the tradition of previous books in the Culture of Specialized Cells Series—each methods and protocols chapter is laid out exactly like the next, with stepwise protocols, preceded by specific requirements for that protocol, and a concise discussion of methods illustrated by data. The editors describe a limited number of representative techniques across a wide spectrum of stem cells from embryonic, newborn, and adult tissue, yielding an all-encompassing and versatile guide to the field of stem cell biology and culture.

The book includes a comprehensive list of suppliers for all equipment used in the protocols presented, with websites available in an appendix. Additionally, there is a chapter on quality control, and other chapters covering legal and ethical issues, cryopreservation, and feeder layer culture. This text is a one-stop resource for all researchers, clinical scientists, teachers, and students involved in this crucial area of study.


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Culture of Human Stem Cells (Culture of Specialized Cells) + Human Stem Cell Technology and Biology: A Research Guide and Laboratory Manual + Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications
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Editorial Reviews

Review

"For those working, or considering working with human stem cells, Culture of Human Stem Cells will be an essential reference." (The Biochemical Society, October 2009)

"Valuable to those entering the field from a wide spectrum of disciplines…an essential textbook for teachers and students who are involved with the therapeutic potential of stem cell research." (SirReadaLot.org, October 2007)

"The principal value of this book lies in the diversity of topics. In addition to chapters on embryonic stem (ES) cells, interesting chapters cover the derivation of multipotential stem cells from mammary tissue, dental pulp, cornea, cord blood, and adipose tissue." (Doody's)

From the Back Cover

The first book to collect the most effective and cutting-edge methods and protocols for deriving and culturing human embryonic and adult stem cells—in one handy resource

This groundbreaking new book follows the tradition of previous books in the Culture of Specialized Cells series—each methods and protocols chapter is laid out exactly like the next, with stepwise protocols, preceded by specific requirements for that protocol, and a concise discussion of methods illustrated by data. The editors describe a limited number of representative techniques across a wide spectrum of stem cells from embryonic, newborn, and adult tissue, yielding an all-encompassing and versatile guide to the field of stem cell biology and culture.

Culture of Human Stem Cells includes a comprehensive list of suppliers for all equipment used in the protocols presented, with Web sites available in an appendix. Additionally, there is a chapter on quality control, and other chapters covering legal and ethical issues, cryopreservation, and feeder layer culture. Culture of Human Stem Cells is a one-stop resource for all researchers, clinical scientists, teachers, and students involved in this crucial area of study.


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Inside This Book (learn more)
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
mammary stem cells, unrestricted somatic stem cells, human corneal endothelial cells, cytometry wash buffer, differential adhesion assay, mouse embryo feeder, rocking mixer, side population cells, germ cell cultures, from bone marrow stroma, human embryonic germ cells, human embryonal carcinoma cell line, limbal epithelial cells, mouse embryonic feeder, dental pulp tissue, stem cell derivation, suturing forceps, cell strainer, stromal stem cells, human embryonic stem cell lines, mammoplasty tissue, knockout serum replacement, pulled glass pipette, discard the supernate, corneal epithelial stem cells
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Cancer Res, Beckman Coulter, Auerbach Copyright, John Wiley, Item Supplier, Eye Res, Isolation of Chondroprogenitor Cells, Cell Biol, Cell Sci, Santa Cruz, Human Articular Cartilage, Sigma Aldrich, Human Synovium, Improved Neubauer, Cell Res, Crystal Violet, New York, Oil Red, Preparation of Umbilical Cord, Oral Biol, Tissue Eng, Bone Miner, Cell Biochem, Cell Dev, European Pharmacopeia
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