Culturing Nerve Cells, Second Edition (Cellular and Molecular Neuroscience) [Hardcover]

Gary Banker (Editor), Kimberly Goslin (Editor)

Book Description

Publication Date: September 11, 1998 | ISBN-10: 0262024381 | ISBN-13: 978-0262024389 | Edition: second edition

Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. This book is in many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. But it also provides an understanding of the principles behind the protocols. In effect the contributors invite you into their labs and provide much of the information you would obtain from such a visit.The authors of the introductory chapters present the nuts-and-bolts principles of growing nerve cells. The authors of the following chapters discuss the culturing of specific cell types. They explain how their experimental goals have shaped their particular cell culture approach and the advantages and disadvantages of the cell culture systems they have developed. They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development.Contributors : Janet Alder, Hannelore Asmussen, Gerard Bain, Gary Banker, Robert W. Baughman, Richard P. Bunge, Ann Marie Craig, Matthew E. Cunningham, Dominique Debanne, Stephen E. Farinelli, Michael F. A. Finley, Gerald D. Fishbach, Beat H. Gähwiler, W.-Q. Gao, Daniel J. Goldberg, Kimberly Goslin, David I. Gottlieb, Lloyd A. Greene, Mary Beth Hatten, Dennis Higgins, James E. Huettner, Kenneth A. Jones, Naomi Kleitman, Raul Krauss, Ronald M. Lindsay, Nagesh K. Mahanthappa, Carol A. Mason, Margot Mayer-Pröschel, R. Anne McKinney, Mary E. Morrison, Mark Noble, David S. Park, Paul H. Patterson, Mu-ming Poo, Richard T. Robertson, Samuel Schacher, Michael M. Segal, Carolyn L. Smith, Nacira Tabti, Scott M. Thompson, Roseann Ventimiglia, Ginger S. Withers, Patrick M. Wood, Min Yao.

Editorial Reviews

About the Author

Gary Banker is Senior Scientist and Director of the Program in Cellular Neurobiology at the Center for Research on Occupational and Environmenttal Toxicology, Oregon Health Sciences University. Kimberly Goslin is Instructor in the Department of Cell Biology at Harvard Medical School.

Product Details

• Hardcover: 666 pages
• Publisher: A Bradford Book; second edition edition (September 11, 1998)
• Language: English
• ISBN-10: 0262024381
• ISBN-13: 978-0262024389
• Product Dimensions: 9.9 x 7.2 x 1.5 inches
• Shipping Weight: 3 pounds (View shipping rates and policies)
• Average Customer Review: 5.0 out of 5 stars  See all reviews (2 customer reviews)
• Amazon Best Sellers Rank: #733,157 in Books (See Top 100 in Books)

Customer Reviews

Most Helpful Customer Reviews

8 of 8 people found the following review helpful:

5.0 out of 5 stars Must-have for all cellular neuro types., May 17, 1999

By 

Steve M. Potter (Atlanta, GA USA) - See all my reviews
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This review is from: Culturing Nerve Cells, Second Edition (Cellular and Molecular Neuroscience) (Hardcover)

This is the Bible for nerve cell culture, with all the secrets, tips and methods you need to culture neurons successfully. Also lots of info to help you decide which system/animal/cell type to study. All the chapters include helpful 'trouble-shooting' sections. The authors are tops in their fields, and the 2nd edition includes many crucial improvements in cell culture techniques, such as viral-mediated transfection.

5.0 out of 5 stars Very practical and convenient, January 18, 2009

By 

D. Krueger (Ohio USA) - See all my reviews
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Amazon Verified Purchase

This review is from: Culturing Nerve Cells, Second Edition (Cellular and Molecular Neuroscience) (Hardcover)

This is not a substitute for practical experience and talking with colleagues who have experience and insight to share. But it is good at providing many details and usuable protocols, down to where they get reagents and materials from (important, since neurons can be pretty sensitive to things you normally wouldn't think a big deal for tissue culture work) to use for culturing neurons.