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Cytokines and Colony Stimulating Factors: Methods and Protocols (Methods in Molecular Biology)
 
 
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Cytokines and Colony Stimulating Factors: Methods and Protocols (Methods in Molecular Biology) [Hardcover]

Dieter Körholz (Editor), Wieland Kiess (Editor)

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Book Description

1588290352 978-1588290359 November 8, 2002 1
Hands-on laboratory experts describe for the novice investigator a host of novel technologies and molecular techniques specifically designed to study cellular immunology and promote its gene therapy applications. Presented in step-by-step detail to ensure ready reproducibility, these protocols range from flow cytometric techniques to detect cytokines and growth factors in different specimens, to methods for generating and expanding dendritic cells and hematopoietic progenitors from different origins. Although the protocols require little previous experience, even highly skilled researchers will augment their skills with many novel timesaving techniques.

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Enormous effort has been spent developing many new laboratory techniques for studying immune cells and the immune system, not only to increase fundamental understanding, but also to procure therapeutic success in a clinical setting. In Cytokines and Colony Stimulating Factors: Methods and Protocols, leading laboratory experts describe for the novice investigator a host of novel technologies and molecular techniques specifically designed to study cellular immunology and promote its gene therapy applications. Presented in step-by-step detail to ensure ready reproducibility, these protocols range from flow cytometric techniques to detect cytokines and growth factors in different specimens, to methods for generating and expanding dendritic cells and hematopoietic progenitors from different origins. Each proven method has been fine-tuned for experimental successs by the investigator who developed it, and includes notes on troubleshooting and avoiding potential pitfalls. Although the protocols require little previous experience, even highly skilled researchers will augment their experimental range with many novel timesaving techniques. Cutting-edge and highly practical, Cytokines and Colony Stimulating Factors: Methods and Protocols provides researchers a carefully evaluated set of powerful tools for successful experimental research on cytokines and their role in gene therapy.

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Inside This Book (learn more)
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
cell isolation kit, primary familial erythrocytosis, independent dendritic cell pathways, tubing prep station, immune gene therapy, cell strainer, reference cell line, cord blood cells, human dendritic cells, combined suicide, permeabilizing solution, saponin buffer, dendritic cell differentiation, blood dendritic cells, suicide gene therapy, resting samples, cytokine gene therapy, nonadherent cells, vaccine generation, repopulating cells, fluorescent parameters, packaging cell line, autologous tumor cells, viral solution, vaccine cells
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Gene Ther, Molecular Biology, Humana Press Inc, Cancer Res, San Diego, Becton Dickinson, Life Technologies, San Jose, Stem Cell Technologies, Miltenyi Biotec, Amersham Pharmacia Biotech, Alexis Corp, New York, Sigma-Aldrich Canada Ltd, Seromed Biochrome, American Association, Bergisch Gladbach, Sigma Chemical, Allergy Clin, Arthritis Rheum, Baxter Healthcare, Blood Activation, Cell Biol, Lake Placid, Thousand Oaks
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