This complete and practical manual on expression measurement using DNA arrays covers the existing methods (from nylon macroarrays to oligonucleotide chips) and includes detailed protocols. It has been written by practising scientists who have experienced the difficulties involved in actually using microarrays, and provides helpful advice and hints on setting up these powerful but sometimes tricky methods. Software, data mining procedures and probable future developments, which should be useful to any practising scientist interested in expression measurement, are also covered in this book.
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4.0 out of 5 stars
perhaps some software has improved since then?,
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This review is from: DNA Microarrays: Gene Expression Applications (Paperback)
Been 6 years since the book came out. But the experimental procedures described in it should still mostly be relevant. Enough details are given, and references to journal papers, that an experimenter can learn and use the microarrays.The text also explains the software and underlying algorithms for analysing results. Some might well have been improved in the intervening years. The ever increasing computational power and cheap computer memory means that more intensive analysis could have emerged, to enhance processing of results.
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Inside This Book
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Key Phrases - Statistically Improbable Phrases (SIPs):
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enzyme colorimetric detection, gene expression matrices, nylon microarrays, hybridisation solution, colorimetric detection method, gene expression matrix, complex probes, oligonucleotide chips, catalyzed reporter deposition, hybridisation signals, gene expression data, colony filters, expression measurement, solid pins, transcription factor binding sites, expression profiles, cannabinoid receptor, differentially expressed genes Key Phrases - Capitalized Phrases (CAPs): (learn more)
Nucleic Acids Res, Proc Natl Acad Sci, Expression Profiler, Genome Res, Boehringer Mannheim, Hum Mol Genet, Fast Red, Immunol Methods, Mol Biol, Nat Genet, Stanford University, Life Technologies, References Alizadeh, Thermal Cycler, United Arab Emirates New!
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enzyme colorimetric detection, gene expression matrices, nylon microarrays, hybridisation solution, colorimetric detection method, gene expression matrix, complex probes, oligonucleotide chips, catalyzed reporter deposition, hybridisation signals, gene expression data, colony filters, expression measurement, solid pins, transcription factor binding sites, expression profiles, cannabinoid receptor, differentially expressed genes Key Phrases - Capitalized Phrases (CAPs): (learn more)
Nucleic Acids Res, Proc Natl Acad Sci, Expression Profiler, Genome Res, Boehringer Mannheim, Hum Mol Genet, Fast Red, Immunol Methods, Mol Biol, Nat Genet, Stanford University, Life Technologies, References Alizadeh, Thermal Cycler, United Arab Emirates New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
This book cites 2
books:
- Computational Molecular Biology, Volume 12 (Topics in Discrete Mathematics) by S. Istrail on page 127
- Artificial Intelligence by A. E. Nisenfedl on page 127
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