DNA Viruses: A Practical Approach groups together the major experimental methods currently employed to study DNA viruses, from the fundamentals of virus culture to novel techniques such as surface plasmon resonance spectrometry and realtime PCR analysis of drug resistance mutations in clinical isolates. Chapter 1 provides an overview of the extraction, purification and characterizations of virus DNA, but also covers the fundamentals of DNA virus culture. Chapters 2 and 3 describe approaches to the molecular investigation and mutagenesis of DNA virus genomes. Chapter 4 considers DNA virus replication and then chapters 5 & 6 describe methods to study transcription control. Chapters 7 to 9 consider aspects of the pathogenesis of DNA virus infections. The final chapter describes the current technology being applied to the development of DNA virus vectors for gene delivery. This volume will therefore be of interest to all those working on DNA viruses whether in academia, industry or clinical research.
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Alan J. Cann is at University of Leicester.
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This review is from: DNA Viruses: A Practical Approach (Paperback)
DNA Viruses: A Practical Approach describes in complete detail all of the major experimental methods currently employed to study DNA viruses, from the fundamentals of virus culture to novel techniques such as surface plasmon resonance spectrometry and real-time PCR analysis of drug resistance mutations. The book begins by describing the culture of DNA viruses and the extraction, purification and characterization of virus DNA. Other topics then covered include virus genome mapping; correlation of genotype with phenotype; sequence analysis; mutagenesis of DNA virus genomes; identification and purification of proteins involved in DNA virus replication; analysis of virus replication at the molecular level; analysis of viral gene transcription and its control; identification and study of trans-acting regulatory proteins; identification, purification and characterization of viral cytokine-binding proteins; analysis of DNA virus proteins involved in neoplastic transformation; the development of DNA virus vectors for gene delivery; chemotherapy of DNA virus infections. This volume is authoritative, comprehensive and fully up-to-date. It is an essential laboratory manual and reference for all those working on DNA viruses whether in academia, industry or clinical research.
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Inside This Book
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First Sentence:
Since viruses will only replicate within living cells, special methods have to be employed for growth in vitro. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
unlabelled cytokine, radiolabelled cytokine, cell culture equipment, occurring restriction sites, complementing cell lines, termination buffer, hundredfold excess, virus oncoproteins, helper cell lines, disruption buffer, cellular supernatants, ultrasonic water bath, trypsinize the cells, indicator viruses, immunoprecipitation buffer, plaque isolates, virus inoculum, permissive cells, adenovirus recombinants, virus stock, marker rescue, fowlpox virus, plaque reduction assay, adenovirus genome, primer walking Key Phrases - Capitalized Phrases (CAPs): (learn more)
Acids Res, Continued Method, Gene Ther, Boehringer Mannheim, Gibco Life Technologies, Oxford University Press, Phosphor Imaging, Fast Flow, New York, Raven Press, Academic Press, Cold Spring Harbor Laboratory Press, Humana Press, Seminars Virol, Flow Laboratories, Giemsa Modified, Micro Columns, Pharmacia Biotech, Repeat Step New!
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Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Since viruses will only replicate within living cells, special methods have to be employed for growth in vitro. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
unlabelled cytokine, radiolabelled cytokine, cell culture equipment, occurring restriction sites, complementing cell lines, termination buffer, hundredfold excess, virus oncoproteins, helper cell lines, disruption buffer, cellular supernatants, ultrasonic water bath, trypsinize the cells, indicator viruses, immunoprecipitation buffer, plaque isolates, virus inoculum, permissive cells, adenovirus recombinants, virus stock, marker rescue, fowlpox virus, plaque reduction assay, adenovirus genome, primer walking Key Phrases - Capitalized Phrases (CAPs): (learn more)
Acids Res, Continued Method, Gene Ther, Boehringer Mannheim, Gibco Life Technologies, Oxford University Press, Phosphor Imaging, Fast Flow, New York, Raven Press, Academic Press, Cold Spring Harbor Laboratory Press, Humana Press, Seminars Virol, Flow Laboratories, Giemsa Modified, Micro Columns, Pharmacia Biotech, Repeat Step New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
This book cites 7
books:
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- Short Protocols in Molecular Biology (2 volume set) by Frederick M. Ausubel on page 208
- Protein Purification Methods: A Practical Approach (Practical Approach Series) by E. L. V. Harris on page 208
- Molecular Cloning: A Laboratory Manual, Third Edition (3 volume set) by Joe Sambrook on page 46
- Cellular Calcium: A Practical Approach (Practical Approach Series) by James G. McCormack on page 208
- Laboratory Manual by Oliver on page 46
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