The ELISA Guidebook (Methods in Molecular Biology) (Spiral-bound)

~ John R. Crowther (Author) "This chapter examines what areas of sciences are needed to allow optimal use of ELISA and notes their relationships..." (more)
Key Phrases: pretitrated system, antispecies conjugate, capture serum, Validation of Diagnostic Tests, Guidebook Table, Theoretical Considerations (more...)

Editorial Reviews

Review

"John R. Crowther has extensive experience in working with ELISA. The book reflects his experience. The graphics and tables are extremely helpful and thoughtfully laid out. All of the ELISA systems are described both in the text and supported by graphical illustrations. Likewise, many of the tables regarding solving problems and troubleshooting are invaluable. Tables summarizing blocking buffers, definitions, and antibody combinations are helpful for quick reference. . .There is no other book of this kind currently in print. The ELISA Guidebook is essential to libraries at any health science research institution since use of this assay is commonplace across a wide variety of disciplines. . .This book would be extremely helpful at any immunodiagnostic company that develops ELISAs. Laboratories that create and use ELISA tests on a regular basis might find this book a useful purchase ."-E-streams Electronic Book Review Journal

"...There is a lot of useful information not present in many other single ELISA publications and so it is the sort of book that is good to have around the lab." -Immunology News

Product Description

John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.

Product Details

• Spiral-bound: 436 pages
• Publisher: Humana Press; 1st edition (August 1, 2000)
• Language: English
• ISBN-10: 0896037282
• ISBN-13: 978-0896037281
• Product Dimensions: 8.8 x 6 x 1.1 inches
• Shipping Weight: 1.6 pounds (View shipping rates and policies)
• Average Customer Review: 3.0 out of 5 stars  See all reviews (1 customer review)
• Amazon.com Sales Rank: #1,119,320 in Books (See Bestsellers in Books)

Customer Reviews

Most Helpful Customer Reviews

5 of 5 people found the following review helpful:

3.0 out of 5 stars Good but annoying, June 25, 2008

By	Erwin H. Abel "James" (CA, USA) - See all my reviews (REAL NAME)

I'm not one for writing reviews but I just had to write this. This book does have useful info but it is horribly written and badly edited. Definitions and explanations on topics are given pages after their introduced. Tables and figures are horribly labeled, if labeled at all. Example I'm currently on pg 226 and table 17 is completely wrong, read the description of the experiment. Another example pages 221 and 222 have identical figures but only one is labeled correctly, page 218 an equation is given which is completely wrong, try it. There are even missing figures. This has got to be one of the most annoying and frustrating texts I have ever read.