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  • Edvotek 335 RT-PCR A Model for the Molecular Biology of HIV Replication
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Edvotek 335 RT-PCR A Model for the Molecular Biology of HIV Replication


List Price: $195.00
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Specifications for this item
Brand Name Edvotek Inc
Number of Items 1
Part Number 335 EDVA7
Size For 6 sets of reactions
UNSPSC Code 60101733

A specific mRNA is reverse transcribed to double-stranded DNA. This DNA product is then amplified... Read full product description


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WARNING:
CHOKING HAZARD -- Small parts. Not for children under 3 yrs.

Product Features

  • For 6 sets of reactions
  • Kit includes: instructions, RNA template, primer mix, RT-PCR reaction beads, RNase-Free water, standard DNA markers, agarose, electrophoresis buffer, InstaStain Ethidium Bromide, gel loading solution
  • You need: thermal cycler, horizontal gel electrophoresis apparatus, power supply, automatic micropipet with tips, balance, microwave oven or hot plate, waterbath
  • All you need: UV transilluminator, pipet pump or bulb, lab glassware, distilled water
  • Reverse transcription 35 min, PCR 120 min, gel prep 30 min, electrophoresis 45 min, staining/destaining 2 min


Product Details

EDVO-Kit #335 Protocol [PDF]| EDVO-Kit #335 Safety Data Sheets (SDS) [PDF]| Material Safety Data Sheet [57kb PDF]

Product Description

A specific mRNA is reverse transcribed to double-stranded DNA. This DNA product is then amplified by PCR. This reaction demonstrates the mode of replication of HIV, which contains reverse transcriptase. This experiment is the first introduction of a commercial RNA experiment for the classroom laboratory. Kit includes: instructions, RNA template, primer mix, RT-PCR reaction beads, RNase-Free water, standard DNA markers, agarose, electrophoresis buffer, InstaStain ethidium bromide and gel loading solution. You need: thermal cycler, horizontal gel electrophoresis apparatus, power supply, automatic micropipet with tips, balance, microwave oven or hot plate, waterbath, UV transilluminator, pipet pump or bulb, lab glassware and distilled water.


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