Plasmid and lambda DNA are pre-digested with restriction enzymes - endonucleases that recognize and cut double-stranded DNA within or near defined base sequences. Digests are separated by agarose gel electrophoresis. Kit includes: instructions, Ready-to-Load DNA samples, UltraSpec-Agarose powder, practice gel loading solution, electrophoresis buffer, InstaStain Blue and FlashBlue stain, calibrated pipet, 100ml graduated cylinder, and microtipped transfer pipettes. All you need: electrophoresis apparatus, power supply, automatic micropipet and tips, balance, microwave or hot plate, visualization system. DNA samples are stable at room temperature. However, if the experiment will not be conducted within one month of receipt, it is recommended that the DNA samples be stored in the refrigerator. DNA samples do not require heating prior to gel loading. Ready-to-Load DNA samples for electrophoresis: A: Plasmid DNA (uncut), B: Plasmid cut with Bgl l, C: Plasmid cut with Eco Rl, D: Lambda DNA (uncut), E: Lambda DNA cut with Eco Rl, F: Lambda DNA cut with Bgl l.