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Human Embryonic Stem Cell Protocols (Methods in Molecular Biology) Hardcover – March 1, 2006

ISBN-13: 978-1588294975 ISBN-10: 1588294978 Edition: 1st

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"The book presents 22 chapters from an international group of experts in the field … . Human Embryonic Stem Cell Protocols is designed to provide a collection of the essential methods in hESC culture and maintenance … . a comprehensive set of valuable protocols from leading research labs in the world. … This book is a valuable resource for hESC investigators who hope to expand their understanding of the manipulation of hESCs." (Elayne Chan and George Q. Daley, Bone Marrow Transplantation, May, 2006)

From the Back Cover

Despite political and ethical controversies surrounding the study of human embryonic stem (hES) cells, new freedoms in regard to using them for research has allowed interest to remain high in understanding the regulatory mechanisms of stem cell self-renewal, their differentiation along various lineages, and their potential use in regenerative medicine. In Human Embryonic Stem Cell Protocols, Second Edition, internationally respected researchers expand upon the popular first edition and describe in detail their most useful techniques for the molecular and cellular manipulation of these intriguing cells. This diverse collection of readily reproducible methods has been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. As a volume of the Methods in Molecular Biology™ series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Human Embryonic Stem Cell Protocols, Second Edition offers both novice and expert researchers powerful tools essential to understanding the maintenance and differentiation of human embryonic stem cells, as well as their applications in regenerative medicine today.

--This text refers to an alternate Hardcover edition.

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