Embryonic Stem Cells: Methods and Protocols (Methods in Molecular Biology) (Methods in Pharmacology and Toxicology) [Hardcover]

Kursad Turksen (Author, Editor)

Book Description

Publication Date: November 1, 2001 | ISBN-10: 0896038815 | ISBN-13: 978-0896038813 | Edition: 1st

Kursad Turksen and a panel of international experts describe their most productive methods for using embryonic stem (ES) cells as in vitro developmental models for many cell and tissue types. Set out in step-by-step detail, these protocols range widely from ES cell isolation, maintenance, and modulation of gene expression, to cutting-edge techniques that use cDNA arrays in gene expression analysis and phage display libraries, the generation of antibodies against very rare antigens, and the identification of and characterization of protein and protein interactions. Embryonic Stem Cells: Methods and Protocols will prove an invaluable resource not only for those interested generally in cell and developmental biology, but also for those actively using, or planning to use ES cells, to study fate choices and specific lineages.

Editorial Reviews

Review

The book could be used effectively by experienced or novice researchers in the field since the protocols are written clearly and concisely. There are 35 brief chapters in this book by over 80 authors who are experienced and knowledgeable researchers in the field...Among the many experimental protocols described in this book are methods for isolating, culturing, and establishing ES cells from a variety of experimental animals. The protocols are written clearly and should serve as a useful laboratory resource for anyone wishing to learn how to culture ES cells and/or apply the specialized techniques for studying them. There is an adequate index. This book is intended and written as a laboratory guide manual. It would be quite useful for anyone doing bench research on ES cells. - Doody's Health Sciences Book Review Journal ...this book provides a good overview of work being done in the development of protocols for culture, controlled differentiation and manipulation of ES cells - Newsletter of the British Society of Developmental Biology Embryonic Stem Cells Methods and Protocols is one of the most useful books I have ever read in this field, and the awesome note at he end of each chapter makes it a great work. It represents good value for money and should be useful as a reference book. - Cell Biology International ...a valuable collection of up-to-date protocols written by leading experts in the field. It is well written and clearly structured. The volume appears highly valuable for any scientist who wants to make a start in this exciting field but also for experienced ES cell researchers who want to widen their repertoire. In addition, the protocols involving hematopoietic, adipocyte and vascular differentiation could easily be adopted by diabetologists to promote our understanding of cell systems which are of special interest in diabetes research. Diabetologia This book provides a very informative resource for any developmental or cell biologist with an interest in developments and prospects of ES cell research. - Molecular Biotechnology

Product Details

• Hardcover: 499 pages
• Publisher: Humana Press; 1st edition (November 1, 2001)
• Language: English
• ISBN-10: 0896038815
• ISBN-13: 978-0896038813
• Product Dimensions: 10.2 x 7.2 x 1.3 inches
• Shipping Weight: 2.8 pounds
• Amazon Best Sellers Rank: #1,980,474 in Books (See Top 100 in Books)