Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used to measure the rate of an enzyme-catalysed reaction is limited only by the nature of the chemical change and the ingenuity of the investigator. This book describes the design and execution of enzyme assays, covering both general principles and specific chapters. Building upon the highly popular first edition, this book combines revised or rewritten chapters with entirely new contributions. Topics include include experimental protocols covering photometric, radiometric, HPLC, and electrochemical assays, along with methods for determining enzyme assays after gel electrophoresis. The theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Also included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high- throughout screening; statistical analysis of enzyme kinetic data; and the determination of active site concentration. This second edition of Enzyme Assays will be valuable not only to biochemists, but to researchers in all areas of the life sciences.
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Provides a very detailed and useful discussion of practical issues in kinetic analysis, illustrates with relevant examples, and provides primary references to the biochemical literature, which are up-to-date ... It is clearly written and illustrated, and will appeal to professional biochemists interested in enzymes. Natural Product Reports
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About the Author
Robert Eisenthal is in the Department of Biology and Biochemistry, University of Bath, UK. Michael Danson is in the Department of Biology and Biochemistry, University of Bath, UK.
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Enzyme Assays Explained,
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This review is from: Enzyme Assays: A Practical Approach (Paperback)
In an age of computerized and automated asay systems, it is possible to gather data without understanding the chemistry or biochemistry involved. This may be suitable for lab techs who work for commercial companies, but it is not a good idea for serious scientists. This book explains how assays work, what can go wrong and what the limitations are. It also reviews the types of instrumentation commonly used in assays. It is an excellent resource for laboratory instructors and graduate students.
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Inside This Book
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The activety of an enzyme may be measured by determining the rate of formation or substrate used during the enzyme-catalysed reaction. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
processed starch gel, diluted electrode buffer, radiometric enzyme assays, respiration medium, imaging beads, blank rate, active site titration, agar solution, gel buffer, contaminating enzyme, initial substrate concentration, progress curve, absorbance coefficient, assay mixture, radiometric assays, coupling enzyme, carbonate dehydratase, coupled assay, replicate observations, extraction medium, cell breakage, assay volume, pure error, true substrate, negative cooperativity Key Phrases - Capitalized Phrases (CAPs): (learn more)
New York, Academic Press Inc, Amersham Pharmacia Biotech, Protocol Add, Methods Enzymol, Protocol Dissolve, Bios Scientific Publishers, Hewlett Packard, Oxford University Press, Absolute Purified, Comparative All, Drug Discovery Today, Nature Biotechnol, Portland Press, Verlag Chemie New!
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The activety of an enzyme may be measured by determining the rate of formation or substrate used during the enzyme-catalysed reaction. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
processed starch gel, diluted electrode buffer, radiometric enzyme assays, respiration medium, imaging beads, blank rate, active site titration, agar solution, gel buffer, contaminating enzyme, initial substrate concentration, progress curve, absorbance coefficient, assay mixture, radiometric assays, coupling enzyme, carbonate dehydratase, coupled assay, replicate observations, extraction medium, cell breakage, assay volume, pure error, true substrate, negative cooperativity Key Phrases - Capitalized Phrases (CAPs): (learn more)
New York, Academic Press Inc, Amersham Pharmacia Biotech, Protocol Add, Methods Enzymol, Protocol Dissolve, Bios Scientific Publishers, Hewlett Packard, Oxford University Press, Absolute Purified, Comparative All, Drug Discovery Today, Nature Biotechnol, Portland Press, Verlag Chemie New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
This book cites 2
books:
11
books
cite this book:
See all 11 books citing this book
- International Business (Spanish Translation) (10th Edition) by John D Daniels on page 101
- Enzymology LabFax by P. C. Engel on page 44
- Enzyme Assays: Essential Data by S. Gul in Back Matter (1), Back Matter (2), and Back Matter (3)
- Proteins LabFax by N. C. Price on page 11, and page 30
- Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (Methods of Biochemical Analysis) by Robert A. Copeland on page 110
- Biochimica industriale: Enzimi e loro applicazioni nella bioindustria (Italian Edition) by R. Verga in Back Matter
- Industrial Biotransformations by Andreas Liese on page 113
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