Practical Enzyme Kinetics provides a practical how-to guide for beginning students, technicians, and non-specialists for evaluating enzyme kinetics using common software packages to perform easy enzymatic analyses.
FREE Two-Day Shipping for students on millions of items. Learn more |
Book Description
Special Offers and Product Promotions
- Buy $50 in qualifying physical textbooks, get $5 in Amazon MP3 Credit. Here's how (restrictions apply)
Editorial Reviews
Review
“…a useful book as an introduction to the topic…complements existing authorative treatments in a concise way...” (Journal of The Science of Food and Agriculture, Vol.85, No.1, 15 January 2005)
"...a concise introduction to enzyme kinetics...recommended..." (Choice, Vol. 40, No. 9, May 2003)
"...extremely useful...particularly...the applications and practical examples to demonstrate the mathematical handling of experimental data..." (Clinical Chemistry, Vol. 49, No. 5, 2003)
"...a valuable companion for everyone seeking a quick overview of the theory of enzyme kinetics." (Angewandte Chemie International Edition , Vol. 42, 2003)
"...a useful manual for the experienced researcher and the developing enzymologist." (Journal of Food Quality, Vol. 26)
"...an innovative approach to the field of enzyme kinetic modeling, model transformation, and model analysis...a valuable reference for practitioners in this field." (SIM News, Vol. 53, No. 3, May/June 2003)
"...presents a new way of looking at an old subject... (Angewandte Chemie, 2003)
"...the approach is quite mathematical and will appeal to the reader versed in mathematics and kinetics..." (ChemBioChem, Vol 4(5), 2003)
From the Back Cover
A modern approach to enzyme kinetics and its applications
As catalysts for the majority of metabolic and biochemical reactions in the body, enzymes are important drug targets as well as useful synthetic catalysts. Enzyme kinetics is the study of the speed of an enzyme-catalyzed reaction and provides useful knowledge that aids in the design of enzyme-based processes. A. G. Marangoni’s Enzyme Kinetics: A Modern Approach provides a practical, how-to guide for students, technicians, and nonspecialists to evaluate enzyme kinetics, using common software packages to perform easy enzymatic analyses.
The treatment of enzyme kinetics in this book is radically different from the way the topic is traditionally covered. Marangoni stresses an understanding of how researchers arrive at models, what the models’ limitations are, and how they can be used in practical ways to analyze enzyme kinetic data. With the advent of computers, linear transformations of models have become unnecessary–Enzyme Kinetics does away with all linear transformations of enzyme kinetic models, advancing the use of nonlinear regression techniques. Marangoni develops new ways to carry out analyses of enzyme kinetic data, particularly in the study of pH effects on catalytic activity and multisubstrate enzymes. Other topics addressed include:
- Tools and techniques of kinetic analysis
- Reversible and irreversible enzyme inhibition
- Multisite and cooperative enzymes
- Immobilized and interfacial enzymes
- Characterization of enzyme stability
Enzyme Kinetics is a handy, innovative resource for practicing researchers in the chemical, pharmaceutical, and food science industries.
Product Details
Would you like to update product info or give feedback on images?
|
More About the Author
Discover books, learn about writers, read author blogs, and more.
Customer Reviews
|
Share your thoughts with other customers:
|
||||||||||||||||||||||
|
Most Helpful Customer Reviews
12 of 14 people found the following review helpful:
3.0 out of 5 stars
Modern?,
By
This review is from: Enzyme Kinetics: A Modern Approach (Hardcover)
The author has a view of how to analyse enzyme kinetic experiments that is very different from mine, but there are many who share his opinions, not mine, and many readers will find this an honourable and useful addition to the bookshelf. What the title means by "modern" is explained in the blurb: "With the advent of computers, linear transformations of models have become unnecessary -- Enzyme Kinetics does away with all linear transformations of enzyme kinetic models, advancing the use of nonlinear regression techniques." As I said, this view is not unique to the author, and those who share it will doubtless be glad to see the double-reciprocal plot and all the others consigned to the dustbin of history.
To me it is a case of throwing out the baby with the bath water. Yes, non-linear regression is a good step forward, but it needs to be applied with discretion, and it is dangerous to forget that even if computers are much better than humans at some tasks, they are worse at others. In particular, humans are much better than computers at noticing anomalies, but to do this they need something to look at, in other words a graph, and it is much easier to recognize an anomaly in the graph where the points ought to fall on a straight line than in one way they should fall on a curve.
2 of 3 people found the following review helpful:
3.0 out of 5 stars
Use all the modern tools or stick with linear,
By moderate user (Kentucky) - See all my reviews
This review is from: Enzyme Kinetics: A Modern Approach (Hardcover)
If you do nonlinear analyses, you must also use residual graphs. They are much better than eyeballing whether a graph is linear or eyeballing if the data seem to fit a curve.
If you cannot do residual graphs, you should not use nonlinear analyses and stick with linear graphs, though even they are more dependable with the addition of a residual graph to check how well the data and equation fit. If you can do residuals, nonlinear analyses are faster, but not necessarily better in many cases. So, the reader should choose whether to use the author's method based on whether it is appropriate for their circumstances.
Share your thoughts with other customers: Create your own review
|
|
Inside This Book
(learn more)
First Sentence:
The stoichiometry of a reaction is the simplest ratio of the number of reactant molecules to the number of product molecules. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
enzyme mass balance, experimental data using nonlinear regression, apparent inactivation rate, enzyme rate data, enzyme coverage, alternate substrate inhibitor, interfacial enzyme, cooperative enzyme, bimolecular activation, substrate concentration plot, substrate concentration curve, different fixed concentrations, pepsin molecule, uninhibited enzyme, irreversible enzyme inhibition, microscopic dissociation constants, catalytic parameters, integrated rate equation, enzyme kinetic data, total enzyme concentration, enzyme kinetic behavior, hyperbolic dependence, microscopic species, decimal reduction time, intrinsic dissociation Key Phrases - Capitalized Phrases (CAPs): (learn more)
Time Figure, Department of Food Science, New York New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
The stoichiometry of a reaction is the simplest ratio of the number of reactant molecules to the number of product molecules. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
enzyme mass balance, experimental data using nonlinear regression, apparent inactivation rate, enzyme rate data, enzyme coverage, alternate substrate inhibitor, interfacial enzyme, cooperative enzyme, bimolecular activation, substrate concentration plot, substrate concentration curve, different fixed concentrations, pepsin molecule, uninhibited enzyme, irreversible enzyme inhibition, microscopic dissociation constants, catalytic parameters, integrated rate equation, enzyme kinetic data, total enzyme concentration, enzyme kinetic behavior, hyperbolic dependence, microscopic species, decimal reduction time, intrinsic dissociation Key Phrases - Capitalized Phrases (CAPs): (learn more)
Time Figure, Department of Food Science, New York New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
This book cites 14
books:
See all 14 books this book cites
- Enzyme Structure and Mechanism by Alan Fersht on page 191, and Back Matter
- Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Wiley Classics Library) by Irwin H. Segel on page 192, and Back Matter
- Biochemical Calculations: How to Solve Mathematical Problems in General Biochemistry, 2nd Edition by Irwin H. Segel in Back Matter
- Catalysis in Chemistry and Enzymology by William P. Jencks in Back Matter
- Principles of Enzymology for the Food Sciences (Food Science and Technology) by John R. Whitaker on page 192
See all 14 books this book cites
Books on Related Topics
(learn more)
|
Tags Customers Associate with This Product(What's this?)Click on a tag to find related items, discussions, and people.
|
Sell a Digital Version of This Book in the Kindle Store
If you are a publisher or author and hold the digital rights to a book, you can sell a digital version of it in our Kindle Store.
Learn more
Customer Discussions
|
This product's forum
Active discussions in related forums
Search Customer Discussions
|
Related forums
|
