A collection of cutting-edge methods to analyze and manipulate epidermal cellprecursors and mature epidermal cells. These protocols cover different methods and models for culturing epidermal cells, for enriching very early epidermal progenitors, and for studying epidermal cell commitment and differentiation both in vitro and in vivo. Topics of special interest include the derivation, characterization, and utility of epidermal stem cells, mature epidermal cells and their characterization, and applications in regenerative medicine. These readily reproducible techniques broaden our understanding of the biology of epidermal cells and of their utility in normal tissue homeostasis and regenerative medicine applications.
Thomas P. Dooley, Ph.D.
Birmingham, Alabama USA
Bi-vocational marketplace minister and author of "Praying Faith" (Destiny Image Pub.), "Hope When Everything Seems Hopeless" (Mall Pub.), and "Half-Truths are Lies" (Mall Pub.).
Founder and President of Path Clearer, Inc., a 501(c)(3) organization focused on "Influencing Nations with Truth" (www.pathclearer.com).
By profession Dr. Dooley is a biomedical research scientist/entrepreneur with 65 scientific publications, in the fields of molecular biology, dermatology, oncology, and pharmacology.





