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Epidermal Cells: Methods and Protocols (Methods in Molecular Biology) (Methods in Molecular Biology Series)
 
 
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Epidermal Cells: Methods and Protocols (Methods in Molecular Biology) (Methods in Molecular Biology Series) [Hardcover]

Kursad Turksen (Editor)

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Hardcover, October 20, 2004 $139.00  
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Epidermal Cells: Methods and Protocols (Methods in Molecular Biology) Epidermal Cells: Methods and Protocols (Methods in Molecular Biology)
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Book Description

1588292673 978-1588292674 October 20, 2004 1
A collection of cutting-edge methods to analyze and manipulate epidermal cellprecursors and mature epidermal cells. These protocols cover different methods and models for culturing epidermal cells, for enriching very early epidermal progenitors, and for studying epidermal cell commitment and differentiation both in vitro and in vivo. Topics of special interest include the derivation, characterization, and utility of epidermal stem cells, mature epidermal cells and their characterization, and applications in regenerative medicine. These readily reproducible techniques broaden our understanding of the biology of epidermal cells and of their utility in normal tissue homeostasis and regenerative medicine applications.

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Editorial Reviews

Review

"The protocols are well written and fully detailed; they combine basic science protocols with informative diagrams illustrating results...will become an invaluable reference guide for laboratory researchers interested in mammalian epidermal cells." Doody's Health Sciences Book Review Journal "For anyone who is planning or is already practically involved in experimental dermatological research, this impressive book is a must in the laboratory." -Acta Dermato-Venereologica  

From the Back Cover

In recent years, our ability to understand and manipulate epidermal cells has increased tremendously, opening significant new possibilities in both basic science research and in regenerative medicine, including would healing and transplantation. Epidermal Cells: Methods and Protocols, Second Edition expands upon the popular first edition by bringing together a panel of experienced basic and clinical researchers to describe in step-by-step detail the powerful methods they have developed and optimized to analyze and manipulate epidermal cell precursors and mature epidermal cells. These protocols cover different methods and models for culturing epidermal cells, for enriching very early epidermal progenitors, as well as for studying epidermal cell commitment and differentiation both in vitro and in vivo. Topics of special interest include the derivation, characterization, and utility of epidermal stem cells, mature epidermal cells and their characterization, and applications in regenerative medicine. The protocols follow the successful Methods in Molecular Biology™ series format, providing introductions to their respective topics, lists of the necessary materials and reagents, and notes on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Epidermal Cells: Methods and Protocols, Second Edition offers experienced and novice investigators alike an invaluable collection of readily reproducible techniques designed to broaden not only our understanding of the biology of epidermal cells, but also their utility in normal tissue homeostasis and regenerative medicine applications. --This text refers to an alternate Hardcover edition.

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More About the Author

Thomas P. Dooley, Ph.D.
Birmingham, Alabama USA

Bi-vocational marketplace minister and author of "Praying Faith" (Destiny Image Pub.), "Hope When Everything Seems Hopeless" (Mall Pub.), and "Half-Truths are Lies" (Mall Pub.).

Founder and President of Path Clearer, Inc., a 501(c)(3) organization focused on "Influencing Nations with Truth" (www.pathclearer.com).

By profession Dr. Dooley is a biomedical research scientist/entrepreneur with 65 scientific publications, in the fields of molecular biology, dermatology, oncology, and pharmacology.

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Inside This Book (learn more)
First Sentence:
The analysis of transgenic or knockout mice with epidermal defects can be enhanced by in vitro studies. Read the first page
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
histocultured skin, gel noodle, keratinocyte media, keratinocyte stem cells, hair follicle stem cells, human filaggrin, single positive controls, keratinocyte medium, secondary antibody control, organotypic cocultures, keratinocyte isolation, embryo powder, epidermal stem cells, contracted collagen gel, keratinocyte growth medium, dip rack, dispase solution, gene correction, desmosomal proteins, epidermal development, keratinocyte lines, see full caption, successful staining, feeder cells, keratinocyte colonies
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Humana Press Inc, Key Words, Cell Biol, Amersham Biosciences, Becton Dickinson, San Diego, Roche Applied Sciences, Cold Spring Harbor Laboratory Press, Capture Kit, Franklin Lakes, New York, Santa Cruz Biotechnology, Fisher Scientific, Palo Alto, Roche Diagnostics, Superfrost Plus, Wako Pure Chemical Industries, Cell Res, Elmer Life Sciences, Molecular Cloning, New England Biolabs, Sigma Chemicals, Cell Sri, Color Plate, Fine Science Tools
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