The Eppendorf UV/Vis BioSpectrometer has a 200 to 830nm wavelength range, up to 4nm spectral bandwidth, a xenon flash lamp, and uses a single-beam to measure the concentration and purity of nucleic acid and protein samples in research labs. Pre-stored methods provide access to concentration determination of nucleic acids; proteins and dye-marked nucleic acids and proteins; and the OD 600 method for determining bacterial density by way of turbidity. User-defined methods provide customization. The 200 to 830nm wavelength range can be adjusted on 1nm increments. The up to 4nm bandwidth is used for routine quality control measurements. The xenon lamp provides an internal standard for wavelength accuracy verification, and the xenon flash lamp and single-beam splitter provide real-time reference beam correction with each lamp flash. The basic and kinetic models have a 12.5 x 12.5mm cuvette shaft that accepts standard cuvettes. The kinetic model has a 20- to 42-degree C temperature control range providing on-board enzyme and substrate kinetics measurements. The 5.7" LCD displays test results and provides access to stored methods. The on-board guided software process minimizes errors and integrated data processing provides on-board data analysis. Software for the kinetics model provides retrospective modification of the time window to perform regression analysis. The instrument provides automatic storage of over 1,000 results to ensure results are retained. Two USB ports and an RS-232 port provide communication with a computer (sold separately).
|Measuring principle||Single-beam absorption spectrophotometer with reference beam|
|Wavelength range||200 to 830nm at 1nm increments|
|Spectral bandwidth||< 4nm|
|Photometric range||0 to 3A at 260nm|
|Cuvette shaft||12.5 x 12.5mm, temperature controlled|
|Absorbance light source||Xenon flash lamp|
|dsDNA concentration range||2.5 to 1,500ng/μL|
|Detector||CMOS photodiode array|
|Results storage||> 1,000 results|
|Data Interfaces||USB stick, USB port for connecting to a PC, RS-232|
|Power requirements||110/240VAC + or - 10%, 50/60Hz|
H is height, the vertical distance from the lowest to highest point; W is width, the horizontal distance from left to right; D is depth, the horizontal distance from front to back.
A spectrophotometer is a device used to measure and analyze aspects of light absorption in liquids and solids. Readings are displayed in digital or analog format. A spectrophotometer can have a handheld body for field use, or a bench-top body for laboratory applications. It may have one or more adapters to enable it to measure surfaces or liquids. A spectrophotometer is used for testing and measuring in anatomy, chemistry, agriculture, process plant, food and beverage making, homeland security, and educational applications.
Eppendorf manufactures instruments for cell manipulation and automated devices for liquid handling, for use in life science research laboratories. The company, found in 1945, is headquartered in Hamburg, Germany.