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Epstein-Barr Virus Protocols (Methods in Molecular Biology)
 
 
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Epstein-Barr Virus Protocols (Methods in Molecular Biology) [Hardcover]

Joanna B. Wilson (Editor), Gerhard H. W. May (Editor)

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Book Description

March 15, 2001 0896036901 978-0896036901 1
Joanna Wilson and Gerhard May have assembled a collection of the key molecular biology protocols used in the analysis of Epstein-Barr virus (EBV), along with a series of valuable immunology, cell biology, and transgenic mouse protocols. These readily reproducible techniques include methods for gene expression with mini-EBV plasmids, for expression analysis by FISH, for EBV detection and quantitation, and for cell proliferation and death assays. In addition, there are EBV-based vectors, an up-to-date map of EBV, a comprehensive table of available latent protein antisera, and assays from in vitro to cell to organ to organism levels. Timely and highly practical, Epstein-Barr Virus Protocols provides powerful tools for elucidating the life cycle of EBV and its host interactions, work that promises the emergence of major new treatments and cures for EBV associated diseases, including several forms of human cancer.

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Editorial Reviews

Review

"Timely and highly practical, Epstein-Barr Virus Protocols provides powerful tools for elucidating life cycle of EBV and its host interactions, work that promises the emergence of major new treatments and cures of EVB-associated diseases, including several forms of human cancer." - Anticancer Research "Overall it is an excellent resource aimed at postgraduate students and young researchers. Protocols are extensively referenced to the primary literature at the end of each chapter." -Today's Life Science "This book provides a well-written and concise account of many of the protocols currently in use for the study of the Epstein-Barr virus and its related diseases. It is comprehensive..." "Both volumes are laid out attractively...and contain much valuable information on the pharmacological aspects of neurotoxicology, as well as state-of-the-art methodology." -Microbiology Today "Leading-edge and forward-looking, Tumor Suppressor Genes in Human Cancer illuminates what is currently known of tumor suppressor genes and their regulation, work that is already beginning to revolutionize, and treatment design." -Tumori "This book is highly recommended for collections serving researchers in the area of molecular biology." -E-Streams

From the Back Cover

The application of recombinant DNA technology to the analysis of Epstein-Barr virus (EBV) is rapidly developing sufficient insight into the virus-host interaction, so that its role in disease pathology is now often discernible and can increasingly be interdicted. In Epstein-Barr Virus Protocols, Joanna Wilson and Gerhard May have assembled a collection of the key molecular biology protocols used in the analysis of Epstein-Barr virus, along with a series of valuable immunology, cell biology, and transgenic mouse protocols. Described in step-by-step detail by experts who use them regularly, these readily reproducible techniques include methods for gene expression with mini-EBV plasmids, for expression analysis by FISH, for EBV detection and quantitation, and for cell proliferation and death assays. In addition, the authors provide information on EBV-based vectors, an up-to-date map of EBV, a comprehensive table of available latent protein antisera, and assays from in vitro to cell to organ to organism levels. Timely and highly practical, Epstein-Barr Virus Protocols provides powerful tools for elucidating the life cycle of EBV and its host interactions, work that promises the emergence of major new treatments and cures for EBV-associated diseases, including several forms of human cancer.

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Inside This Book (learn more)
First Sentence:
This chapter summarizes the genes and mRNAs that have been mapped on to the B95-8 EBV genome. Read the first page
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
cell fixation solution, oncogene product latent membrane protein, mixture lacking tryptophan, buffered trypsin, chemical carcinogen treatment, collagen rafts, pronase concentration, standard cytotoxicity assay, viral nuclear antigen, centrifuge yeast, primary epithelial cells, dispase solution, complete tissue culture medium, bait vector, magnetic enrichment, latent proteins, virus nuclear antigen, resuspend yeast, blotting buffer, virus latent membrane protein, cleavage buffer, strain recognition, complete growth medium, new fuchsin, binding buffer containing
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Epstein-Barr Virus Protocols Edited, Humana Press Inc, Cold Spring Harbor, Nucleic Acids Res, Boehringer Mannheim, Cancer Res, Cell Biol, New York, Miltenyi Biotec, Alamar Blue, Genes Dev, Generating Transgenic Mice, Laboratory Manual, Trends Microbiol, Virus Plasmids, Kinases Regulated, Amersham Pharmacia, Becton Dickinson, Current Protocols, Introduction Epstein-Barr, Introduction of Plasmid Vectors, Maria Masucci, Perkin Elmer, Phenotype Determination, San Diego
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