The Expanding Role of Mass Spectrometry in Biotechnology [Paperback]

Gary Siuzdak (Author)

Book Description

Publication Date: August 1, 2003 | ISBN-10: 0974245100 | ISBN-13: 978-0974245102

The Expanding Role of Mass Spectrometry in Biotechnology covers the basic concepts in mass spectrometry as well as advanced topics including protein identification/protein structural analysis, carbohydrate and oligonucleotide analysis. Topics also include pharmacokinetics, high throughput screening, and the recent development of mass spectrometry in clinical diagnosis.

Editorial Reviews

About the Author

Gary Siuzdak obtained his Ph.D. at Dartmouth College and is currently at The Scripps Research Institute where he is Director of the Center for Mass Spectrometry and Associate Professor of Molecular Biology.

Product Details

• Paperback: 288 pages
• Publisher: MCC Press (August 1, 2003)
• Language: English
• ISBN-10: 0974245100
• ISBN-13: 978-0974245102
• Product Dimensions: 8.9 x 6 x 0.7 inches
• Shipping Weight: 1 pounds
• Average Customer Review: 5.0 out of 5 stars  See all reviews (5 customer reviews)
• Amazon Best Sellers Rank: #913,277 in Books (See Top 100 in Books)

Customer Reviews

Most Helpful Customer Reviews

4 of 4 people found the following review helpful:

5.0 out of 5 stars An essential, well-written text for Mass Spec and Biotech, September 16, 2003

By 

Joanna Daneman (Middletown, DE USA) - See all my reviews
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This review is from: The Expanding Role of Mass Spectrometry in Biotechnology (Paperback)

Mass spectroscopy is one of the most useful techniques in biotechnology, especially proteomics. The identification of drug metabolites, synthesis products and protein and protein fragments is a central activity in many types of research projects. Small molecules and larger macromolecules can be analyzed, both for molecular weight and their structure. The popularity of mass spectrometric analysis in biotechnology has soared over the last decade.

There have been new developments in mass spec, including hybrid instruments that combine MALDI (Matrix-assisted laser desorption) with ESI electrospray, new ion trap instruments, and many developments such as high-throughput analysis and mass spec for clinical diagnostics. This book serves as both an update to Dr. Siuzdak's first book "Mass Spectrometry for Biotechnology" and really, serves as a replacement since the original text was published in 1996.

The chapters include:
1. Ionization and the Mass Spectrometer
2. Mass Analyzers
3. Practical Aspects of Biomolecular Analysis
4. Peptide and Protein Analysis
5. Protein Profiling
6. Protein Structure characterization
7. Nucleic acid, carbohydrates and steroids
8. High throughput analysis
9. Pharmacokinetics
10. Mass Spectrometry in Action (applications)

For instructors in analytical chemistry courses at the college or graduate level, the book is a useful text with questions at the end of the chapters to be used as review. These are not in-depth questions, but they are a good tool for the self-directed student to check his or her comprehension. The later chapters are heavy on applications such as carbohydrate and oligonucleotide and steroid analysis, as well as pharmacokinetics. There are many illustrations, well-drawn and clear. This compact, well-written and useful primer should be on the shelf of anyone doing mass spec in biotechnology, anyone studying analytical chemistry or really, anyone in biotechnology.

3 of 3 people found the following review helpful:

5.0 out of 5 stars Empower your biological research, January 27, 2004

By 

Richard Kriwacki (Memphis, TN USA) - See all my reviews

This review is from: The Expanding Role of Mass Spectrometry in Biotechnology (Paperback)

Mass spectrometry is playing an increasingly important role in biological research due to its extremely high sensitivity, incredible resolving power and compatibility with high throughput assays. The prominent position of mass spectrometry today is due in part to fast paced technical innovations that have resulted in a range of highly sensitive and versatile instruments. The newest version of the Siuzdak text, "The Expanding Role of Mass Spectrometry in Biotechnology", distills this challenging topic to its essence, allowing scientists and students alike to understand the basic concepts and essential requirements of the most commonly applied mass spectrometry techniques. Mastery of this well presented and easily read material will empower biologists to expand their research horizons. This text deserves a spot on every biomedical researcher's bookshelf.

2 of 2 people found the following review helpful:

5.0 out of 5 stars Will it be translated into Chinese ?, November 6, 2005

By 

Yang Min (China) - See all my reviews

This review is from: The Expanding Role of Mass Spectrometry in Biotechnology (Paperback)

After reading "The Expanding Role of Mass Spectrometry in Biotechnology", I believe it should be recommended to more students including undergraduates and graduate students majoring in analytical chemistry, organic chemistry, biology, or related areas. And it would be a helpful reference for students who have some basic knowledge about MS and would like to learn more about its current development and applications. For myself as a graduate student in analytical chemistry it concisely explained and summarized the different areas of MS and provided a comprehensive view on its development in bioanalytical science. Moreover, it is a good text book as it is easy to understand and the questions at the end of each chapter are good guides for students to master key pionts. Last month, I used this book to educate my roomate, a PhD candidate in organic chemistry, on the ionization principles of ESI. The great images and examples helped her a lot . So, the question is... will Gary Siuzdak translate it into Chinese to make it more easily available to other Chinese students!