Peter E. Vaillancourt presents a collection of popular and emerging methodologies that take advantage of E. coli's ability to quickly and inexpensively express recombinant proteins. The authors focus on two areas of interest: the use of E. coli vectors and strains for production of pure, functional protein, and the use of E. coli as host for the functional screening of large collections of proteins and peptides. Among the cutting-edge techniques demonstrated are those for rapid high-level expression and purification of soluble and functional recombinant protein and those essential to functional genomics, proteomics, and protein engineering.
E. coli Gene Expression Protocols
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Editorial Reviews
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"...many will find it extremely useful to have this book on hand when trying to achieve high levels of pure, soluble recombinant protein; or incorporating an E. coli expression step into the procedures of functional genomics, proteomics and protein engineering." - Microbiology Today
From the Back Cover
Gene expression using E. coli as a host is carried out over a wide range of disciplines in academic and industrial laboratories. In E. coli Gene Expression Protocols, Peter E. Vaillancourt presents a collection of popular and emerging methodologies that take advantage of E. coli's ability to quickly and inexpensively express recombinant proteins. The authors focus on two areas of interest: the use of E. coli vectors and strains for production of pure, functional protein, and the use of E. coli as host for the functional screening of large collections of proteins and peptides. Among the cutting-edge techniques demonstrated are those for rapid high-level expression and purification of soluble and functional recombinant protein, and those essential to functional genomics, proteomics, and protein engineering. Described in step-by-step detail to ensure robust, trouble-free results, each proven method has been written by a hands-on expert and includes extensive notes and practical tips for avoiding pitfalls. Even highly skilled researchers will find many time-saving techniques. Authoritative and highly practical, E. coli Gene Expression Protocols provides a state-of-the-art collection of tested methods for this powerful gene expression technology, offering today's investigators proven tools for success in the emerging fields of functional genomics and proteomics.
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Inside This Book
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First Sentence:
Rapid transfer of exponentially growing E. coli cultures from physiological to low temperatures (10-15 C) has profound consequences on cell physiology: membrane fluidity decreases, which interferes with transport and secretion, the secondary structures of nucleic acids are stabilized, which affect the efficiencies of mRNA transcription/translation and DNA replication, and free ribosomal sub-units and 70S particles accumulate at the expense of polysomes, negatively impacting translation of most cellular mRNAs (1-3). Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
protein ligand solution, solubility probability, calmodulin affinity matrix, chitin resin, consecutive rare codons, filter washing buffer, engineered inteins, thioredoxin fusion proteins, automated pipetting system, dual expression vector, clarified cell extract, different inteins, structural complementation, modified intein, repressor fusions, passenger protein, chemically competent cells, carotenogenic genes, chitin column, protein ligation, chitin beads, downshift temperature, folding modulators, cleavage buffer, transcriptional protein Key Phrases - Capitalized Phrases (CAPs): (learn more)
Cold Spring Harbor, Gene Expression Protocols Edited, Humana Press Inc, New England Biolabs, New York, Nucleic Acids Res, Multifunctional Fusion Tag, Cold-Inducible Promoters, Solubility Enhancement, Protein Expr, Enhance Solubility, Methods Enzymol, Protein Sci, New Fusion Proteins, Trends Biochem, Biosynthesis of Novel Carotenoids, John Wiley, Amp Kan, Co-Expression of Proteins, Prolinx Inc, Amersham Pharmacia, Cloning System, Genetics Institute, Nature Biotechnol, Palo Alto New!
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Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Rapid transfer of exponentially growing E. coli cultures from physiological to low temperatures (10-15 C) has profound consequences on cell physiology: membrane fluidity decreases, which interferes with transport and secretion, the secondary structures of nucleic acids are stabilized, which affect the efficiencies of mRNA transcription/translation and DNA replication, and free ribosomal sub-units and 70S particles accumulate at the expense of polysomes, negatively impacting translation of most cellular mRNAs (1-3). Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
protein ligand solution, solubility probability, calmodulin affinity matrix, chitin resin, consecutive rare codons, filter washing buffer, engineered inteins, thioredoxin fusion proteins, automated pipetting system, dual expression vector, clarified cell extract, different inteins, structural complementation, modified intein, repressor fusions, passenger protein, chemically competent cells, carotenogenic genes, chitin column, protein ligation, chitin beads, downshift temperature, folding modulators, cleavage buffer, transcriptional protein Key Phrases - Capitalized Phrases (CAPs): (learn more)
Cold Spring Harbor, Gene Expression Protocols Edited, Humana Press Inc, New England Biolabs, New York, Nucleic Acids Res, Multifunctional Fusion Tag, Cold-Inducible Promoters, Solubility Enhancement, Protein Expr, Enhance Solubility, Methods Enzymol, Protein Sci, New Fusion Proteins, Trends Biochem, Biosynthesis of Novel Carotenoids, John Wiley, Amp Kan, Co-Expression of Proteins, Prolinx Inc, Amersham Pharmacia, Cloning System, Genetics Institute, Nature Biotechnol, Palo Alto New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
This book cites 36
books:
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- Molecular Cloning: A Laboratory Manual, Third Edition (3 volume set) by Joe Sambrook on page 51, and page 116
- Recombinant Antibodies by Frank Breitling on page 301
- Nucleic Acids (Methods in Molecular Biology) by John M. Walker on page 232
- Biostatistical Methods (Methods in Molecular Biology) by Stephen W. Looney in Front Matter
- Quantitative Trait Loci: Methods and Protocols (Methods in Molecular Biology) by Nicola J. Camp in Front Matter
See all 36 books this book cites
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