Leading bench-top experimentalists from around the world present, in a proven format, their state-of-the-art methods for probing the extracellular matrix (ECM), its mechanisms of assembly and how it influences cell behavior. These carefully detailed biochemical, biophysical, genetic, molecular biological, and cell biological techniques have been optimized for the study of ECM structure and assembly, the mechanisms of cell-ECM interactions, and the molecular genetics of ECM proteins and defects in human disease, and bring to a wider audience a range of highly sophisticated methods that have hitherto been difficult even for a few specialized laboratories. Cutting-edge and highly practical, Extracellular Matrix Protocols offers today's ECM researchers a gold-standard collection of powerful, readily reproducible methods to investigate the extracellular matrix and the altered cell-ECM interactions that play so important a role in human health and disease.
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Editorial Reviews
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"This would be most useful to a lab researcher who had not previously been studying the role of the ECM in biological systems, and who wished to do so. Having all the practical protocols for preparing, assaying, and studying these important molecules in a small volume could be very useful for such a researcher."-Doody's Health Science Book Review Journal
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First Sentence:
This chapter will describe the preparation and use of a semipermeabilized (SP) cell system that reconstitutes the initial stages in the assembly and modification of proteins entering the secretory pathway (1). Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
transmigrated cells, vibratome tray, semipermeabilized cells, gelified medium, agarose drop, feeder medium, spreading assays, human prolyl, cartilage constructs, mammary rudiment, gel fixing solution, microplate fluorometer, supported planar bilayers, nitrocellulose disks, fibronectin modules, packaging cells, attachment assays, plasmin digestion, avian osteoclasts, feeder cells, collagen crosslinks, selection cassette, transfection kit, migration assays, retroviral delivery Key Phrases - Capitalized Phrases (CAPs): (learn more)
Extracellular Matrix Protocols Edited, Humana Press Inc, Cell Biol, Cold Spring Harbor, Life Technologies, New York, Agar Scientific, San Diego, Cell Res, Boehringer Mannheim, Interactions Between Proteins, Molecular Probes, Organotypic Tissue Slices, Reconstitution of Functional Integrin, Labeling of Fibronectin Modules, Oligodendrocyte Precursor Migration Assays, Recombinant Collagen Trimers, Corning Costar, Neuroepithelial Differentiation, Tissue Recombinants, Cancer Res, Cell Sci, Manipulating the Mouse Embryo, Nature Genet, Oxford University Press New!
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Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
This chapter will describe the preparation and use of a semipermeabilized (SP) cell system that reconstitutes the initial stages in the assembly and modification of proteins entering the secretory pathway (1). Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
transmigrated cells, vibratome tray, semipermeabilized cells, gelified medium, agarose drop, feeder medium, spreading assays, human prolyl, cartilage constructs, mammary rudiment, gel fixing solution, microplate fluorometer, supported planar bilayers, nitrocellulose disks, fibronectin modules, packaging cells, attachment assays, plasmin digestion, avian osteoclasts, feeder cells, collagen crosslinks, selection cassette, transfection kit, migration assays, retroviral delivery Key Phrases - Capitalized Phrases (CAPs): (learn more)
Extracellular Matrix Protocols Edited, Humana Press Inc, Cell Biol, Cold Spring Harbor, Life Technologies, New York, Agar Scientific, San Diego, Cell Res, Boehringer Mannheim, Interactions Between Proteins, Molecular Probes, Organotypic Tissue Slices, Reconstitution of Functional Integrin, Labeling of Fibronectin Modules, Oligodendrocyte Precursor Migration Assays, Recombinant Collagen Trimers, Corning Costar, Neuroepithelial Differentiation, Tissue Recombinants, Cancer Res, Cell Sci, Manipulating the Mouse Embryo, Nature Genet, Oxford University Press New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
This book cites 28
books:
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43
books
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- Nuclear Magnetic Resonance (Cambridge Monographs on Physics) by Andrew on page 57
- Cell Biology (Molecular and Cell Biochemistry Series) by C. A. Smith in Front Matter
- Lipoprotein Protocols (Methods in Molecular Biology) by Jose M. Ordovas in Front Matter
- Cell Biology by Edwards on page 357
- Receptor Binding Techniques (Methods in Molecular Biology (Cloth)) by Mary Keen in Front Matter
See all 28 books this book cites
- Calpain Methods and Protocols (Methods in Molecular Biology) by John S. Elce in Front Matter
- Mycotoxin Protocols (Methods in Molecular Biology) by Mary W. Trucksess in Front Matter
- Glycoprotein Methods & Protocols The Mucins (Methods in Molecular Biology) by Anthony P. Corfield in Front Matter
- DNA Sequencing Protocols (Methods in Molecular Biology) by Colin A Graham in Front Matter
- T Cell Protocols: Development and Activation (Methods in Molecular Biology) by Kelly P. Kearse in Front Matter
See all 43 books citing this book
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