This new edition of Fluorescent Proteins presents current applications of autofluorescent proteins in cell and molecular biology authored by researchers from many of the key laboratories in the field. Starting from a current review of the broad palette of fluorescent proteins available, several chapters focus on key autofluorescent protein variants, including spectral variants, photodynamic variants as well as chimeric FP approaches. Molecular applications are addressed in chapters that detail work with single molecules, approaches to generating protein fusions and biosensors as well as analysis of protein-protein interactions in vivo by FRET, fluorescence polarization and fluorescence cross corrlation techniques. A number of approaches to in vivo dynamics are presented, including FRAP, photoactivation, and 4-dimensional microscopy. Behavior of spindle components, membrane proteins, mRNA trafficking as well as analysis of cell types in tissues and in development are detailed and provide models for a wide variety of experimental approaches. In addition, several chapters deal directly with the computational issues involved in processing multidimensional image data and using fluorescent imaging to probe cellular behavior with quantitative modeling. This volume brings together the latest perspective and techniques on fluorescent proteins and will be an invaluable reference in a wide range of laboratories.
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bimolecular fluorescence complementation analysis, transposon tagging, biomedical imaging, glycerol stocks, level set method, molecular imaging, fluorescent protein fragments, membrane trafficking assays, bimolecular fluorescent complexes, neuronal differentiation cascade, exclusive interaction partners, alternative interaction partners, confocal stereology, photoactivatable fluorescent proteins, bleached fraction, fluorescent complex formation, multicell images, biosynthetic traffic, transposon lands, cells using bimolecular fluorescence complementation, biosynthetic membrane traffic, computational image analysis, quantitative image data, transgenic reporter lines, nascent blood vessels Key Phrases - Capitalized Phrases (CAPs): (learn more)
Cell Biol, New York, San Diego, Computational Analysis of Dynamic Image Data, The Zebrafish, Ralf Dahm, Plant Cell, Adult Brain, Counting Kinetochore Protein Numbers, Cell Sci, Elsevier Academic Press, N-terminus Amp, Ratiometric Membrane Trafficking Assays, William Detrich, Marketa Samalova, Cancer Res, Robert Mealer, Analysis of Molecular Binding, Louis Hodgson, Invitrogen Cat, Advanced Techniques, Nucleic Acids Res, Methods Enzymol, Oxford University Press, Plant Physiol Browse Sample Pages:
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bimolecular fluorescence complementation analysis, transposon tagging, biomedical imaging, glycerol stocks, level set method, molecular imaging, fluorescent protein fragments, membrane trafficking assays, bimolecular fluorescent complexes, neuronal differentiation cascade, exclusive interaction partners, alternative interaction partners, confocal stereology, photoactivatable fluorescent proteins, bleached fraction, fluorescent complex formation, multicell images, biosynthetic traffic, transposon lands, cells using bimolecular fluorescence complementation, biosynthetic membrane traffic, computational image analysis, quantitative image data, transgenic reporter lines, nascent blood vessels Key Phrases - Capitalized Phrases (CAPs): (learn more)
Cell Biol, New York, San Diego, Computational Analysis of Dynamic Image Data, The Zebrafish, Ralf Dahm, Plant Cell, Adult Brain, Counting Kinetochore Protein Numbers, Cell Sci, Elsevier Academic Press, N-terminus Amp, Ratiometric Membrane Trafficking Assays, William Detrich, Marketa Samalova, Cancer Res, Robert Mealer, Analysis of Molecular Binding, Louis Hodgson, Invitrogen Cat, Advanced Techniques, Nucleic Acids Res, Methods Enzymol, Oxford University Press, Plant Physiol Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Surprise Me!
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