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To use the fluorescence lifetime as a contrast parameter for microscopy instead of the fluorescent intensity has a number of advantages: (i) The fluorescence lifetime of a probe molecule often differs considerably from that of the background radiation or autofluorescence, providing an opportunity to suppress the contributions of the latter to the total signal; (ii) Fluorescence lifetime imaging provides an alternative to the technique of ratio imaging based on wavelength differences which is used for e.g. the imaging of ion concentrations in biological samples.
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Key Phrases - Statistically Improbable Phrases (SIPs):
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lines phase residual, acinar pathways, icosahedral particle orientations, unbranched regions, distal derivative, shear force image, lateral root initiation, large optical system, thick biological specimens, endodermal precursor, meadow moth, central section theorem, confocal scanning optical microscope, microsomal glutathione transferase, coherent transfer function, lateral root primordia, spectral jumps, fractal graphics, sine condition, ice contamination, orientation refinement, cutoff spatial frequencies, orange granules, fluorescence lifetime imaging, tilt series
Key Phrases - Capitalized Phrases (CAPs):
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New York, Plenum Press, Cell Biol, Academic Press, Republic of China, Silicon Graphics, University of Sydney, Applied Optics, Journal of Microscopy, Modern Optics, National Institutes of Health, Surface Science, Zoological Studies, Biomedical Computing, Cosmochimica Acta, Double Pulse Fluorescence Lifetime Imaging, Genes Development, Molecular Probe, Pacific Northwest National Laboratory, Pergamon Press, Plankton Res, Plant Cell, University Press of New England
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