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Forensic DNA Typing Protocols (Methods in Molecular Biology)
 
 
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Forensic DNA Typing Protocols (Methods in Molecular Biology) [Hardcover]

Angel Carracedo (Editor)

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Book Description

November 30, 2004 1588292649 978-1588292643 1
A state-of-the-art collection of readily reproducible laboratory methods for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. For forensic geneticists, there are readily reproducible methods for species identification, ancient DNA, and pharmacogenetics. Additional chapters address new applications in the forensic genetics lab, such a species identification or typing of CYP polymorphisms for the analysis of adverse to drugs.

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From the Back Cover

In recent years, the rapid emergence of different markers and technologies for DNA typing has transformed the field of forensic identification with breathtaking speed. In Forensic DNA Typing Protocols, leading forensic geneticists from around the world describe in step-by-step detail the cutting-edge laboratory methods they now use for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. Additional chapters address new applications in the forensic genetics lab, such a species identification, ancient DNA, or typing of CYP polymorphisms for the analysis of adverse to drugs. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Up-to-date and highly practical, Forensic DNA Typing Protocols captures and makes practical for all laboratories the whole array of new technologies and techniques that have revolutionized forensic science.

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Inside This Book (learn more)
First Sentence:
Deoxyribonucleic acid (DNA) profiling, or typing, as it is now known, was first described in 1985 by Alec Jeffreys and co-workers (7), and it has had a tremendous impact in forensic genetics. Read the first page
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
minisequencing products, pyrosequencing system, loader protocol, minisequencing primers, minisequencing reaction, coding region fragments, reporter pair, multiplex kit, genetic analyser, heterozygote control, forensic genetics, hone sample, different amplicons, allelic ladders, plate document, forensic casework, photocleavable linker, reader protocol, melting curve analysis, amplification plot, typing protocol, large multiplexes, reaction volume containing, allele ranges, multiplex reaction
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Applied Biosystems, Forensic Sri, Legal Med, Map Viewer, Foster City, New England Biolabs, Genetic Analyzer, Nucleic Acids Res, Sequence Viewer, Genome Res, Bruker Daltonik, Plate Low, Tay Gold, Amersham Biosciences, Genuine Res, Roche Diagnostics, Roche Molecular Biochemicals, Unknown Undel, Xunta de Galicia, Applied Biosvstems, Denaturation Annealing Extension Initial, Forensic Sci, Gold Butter, Introduction Single, Life Science
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