This new edition is almost a completely new text, with eight of the ten chapters written by new authors. It presents the most reliable methods for essential procedures such as one-dimensional polyacrylamide gel electrophoresis, isoelectric focusing, two-dimensional gel electrophoresis, preparative gel electrophoresis, and peptide mapping, complete with the latest refinements of the procedures. In addition, it describes major new techniques developed since the previous edition. These include capillary gel electrophoresis, sequence analysis of gel-resolved proteins, fluorophore-labelled saccharide electrophoresis, and analysis of protein-protein interactions by gel electrophoresis. Like the previous editions, this volume is easy-to-follow and thorough, a laboratory manual written by experienced researchers for researchers. The emphasis is on describing the best methods, in step-by-step detail, with copious advice to ensure that each method works the first time. This new edition is a 'must' for anyone currently uses gel electrophoresis.
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Editorial Reviews
Review
"A laboratory manual presenting methods in a step-by-step format, and hints and tips from experienced researchers. Among the varieties of gel electrophoresis described are one-dimensional polyacrylamide; isoelectric, two-dimensional; and preparative. Eight of the ten chapters of the earlier editions, in 1981 and 1990, have been rewritten by new authors. They discuss methods that have been developed and tested since then, including capillary gel electrophoresis, the sequence analysis of gel-resolved proteins, fluorophore-labelled saccharide electrophoresis, and analyzing interactions between proteins."--SciTech Book News
About the Author
B. D. Hames is at University of Leeds.
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2 of 9 people found the following review helpful:
3.0 out of 5 stars
a great resource book,
By A Customer
This review is from: Gel Electrophoresis of Proteins: A Practical Approach (Paperback)
Although there has been many books on gel electophroresis, this book seems to stand out the most. Being a novice in this field, I greatly appreciated Hames' and Rickwoods' practical procedures and methods. I srtongly recommend this book to all scientists and upcoming scientists.
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Inside This Book
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First Sentence:
With the approach of the post-genome era and the popularity of recombinant DNA technology, there has been a resurgence in the use of polyacrylamide gel electrophoresis to identify and characterize various gene products. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
uniform concentration gels, photopolymerization system, sodium toluene sulfinate, stacking gel mixture, centrifugal vacuum evaporator, resolving gel mixture, associated vacuum pump, gel mould, labelled saccharides, resolving gel buffer, acidic saccharides, continuous buffer system, proteinase solution, sample polypeptides, mapping gel, diphenyliodonium chloride, blue staining solution, slab gel apparatus, stacking gel buffer, solubilization solution, repel silane, neutral saccharides, bait protein, gel additives, electrophoresis running buffer Key Phrases - Capitalized Phrases (CAPs): (learn more)
Academic Press, New York, San Diego, Beckman Instruments, Boca Raton, Prep Cell, Mass Spectrom, Bio-Rad Model, Merck Ltd, Boehringer Mannheim, Jouan Ltd, Van Damme, Dextra Laboratories Ltd, Nucleic Acids Res, Oxford Glycosystems Ltd, Urea Method, Van Keuren, Janssen Life Sciences Products New!
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Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
With the approach of the post-genome era and the popularity of recombinant DNA technology, there has been a resurgence in the use of polyacrylamide gel electrophoresis to identify and characterize various gene products. Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
uniform concentration gels, photopolymerization system, sodium toluene sulfinate, stacking gel mixture, centrifugal vacuum evaporator, resolving gel mixture, associated vacuum pump, gel mould, labelled saccharides, resolving gel buffer, acidic saccharides, continuous buffer system, proteinase solution, sample polypeptides, mapping gel, diphenyliodonium chloride, blue staining solution, slab gel apparatus, stacking gel buffer, solubilization solution, repel silane, neutral saccharides, bait protein, gel additives, electrophoresis running buffer Key Phrases - Capitalized Phrases (CAPs): (learn more)
Academic Press, New York, San Diego, Beckman Instruments, Boca Raton, Prep Cell, Mass Spectrom, Bio-Rad Model, Merck Ltd, Boehringer Mannheim, Jouan Ltd, Van Damme, Dextra Laboratories Ltd, Nucleic Acids Res, Oxford Glycosystems Ltd, Urea Method, Van Keuren, Janssen Life Sciences Products New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
Citations (learn more)
This book cites 1
book:
49
books
cite this book:
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- Protein Structure (Carolina Biology Readers) by D. C. Phillips in Front Matter
- Protein Methods by Daniel M. Bollag on 6 pages
- Enzyme Purification and Related Techniques, Part C, Volume 104: Volume 104: Enzyme Purification and Related Techniques Part C (Enzyme Purification & Related Techniques) by Nathan P. Kaplan on 6 pages
- Guide to Protein Purification (Methods in Enzymology, Vol. 182) by Murray P. Deutscher on 4 pages
- Protein Analysis and Purification: Benchtop Techniques by Ian M. Rosenberg on page 115, page 117, and page 209
- Introduction to Macromolecular Science by Petr Munk on page 436, page 437, and page 459
See all 49 books citing this book
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