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Gene Probes: Principles and Protocols (Methods in Molecular Biology)
 
 
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Gene Probes: Principles and Protocols (Methods in Molecular Biology) [Hardcover]

Marilena Aquino do Muro (Editor), Ralph Rapley (Editor)

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Book Description

0896038858 978-0896038851 September 20, 2001 1
Senior scientists Marilena Aquino de Muro and Ralph Rapley have brought together an outstanding collection of time-tested protocols for designing and using genes probes in a wide variety of applications. The applications covered range from searching for specific genes in the human chromosome to the detection of microorganisms and their toxins in the environment and in food samples, as well as in the diagnosis of human disease. Helpful tutorials explain the principles of gene probe design, labeling, detection, target formation, and hybridization conditions.

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Editorial Reviews

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"In this book, the editors have brought together a comprehensive collection of time-tested protocols for designing and using gene probes in a wide variety of applications." -Clinical Laboratory International

From the Back Cover

The use of nucleic acid or gene probes as cloning or diagnostic tools has promoted significant advances in human genome mapping and in the isolation and manipulation of genes generally. In Gene Probes, Marilena Aquino de Muro and Ralph Rapley have brought together an outstanding collection of time-tested protocols for designing and using gene probes in a wide variety of applications. The applications covered range from searching for specific genes in the human chromosome to the detection of microorganisms and their toxins in the environment and in food samples, as well as in the diagnosis of human disease. Each cutting-edge method includes practical hints and tips and is described in step-by-step detail by an expert who not only routinely uses the technique, but has also optimized it for experimental success. Helpful tutorials explain the principles of gene probe design, labeling, detection, target formation, and hybridization conditions. Comprehensive and versatile, Gene Probes: Principles and Protocols provides a cutting-edge practical resource for all biochemists, microbiologists, and molecular biologists who are using this powerful new technology to advance medical, environmental, and food research today.

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Inside This Book (learn more)
First Sentence:
The isolation of specific regions within the genome of an organism is now normally accomplished by polymerase chain reaction (PCR) amplification using primers specific for the region in question. Read the first page
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
hot start modification, human bladder tumors, amplifying solution, acid detection kit, genital precancers, subgenus level, malignant plasma cells, other salmonellae, sterile stock solutions, saline sodium citrate, cholerae strains, formamide concentration, hybridization buffer, gene probes, resuspension buffer, prehybridization solution, casting tray, neutralizing solution, denaturing solution, nuclear fast red, hybridization oven, tubulin genes, probe specificity, target format, blot apparatus
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Boehringer Mannheim, Humana Press Inc, New York, New World Leishmania, Cold Spring Harbor, Nucleic Acids Res, Roche Molecular Biochemicals, Amersham Pharmacia Biotech, Perkin Elmer, Detection of Polychlorinated Biphenyl-Degrading Organisms, Cancer Res, Enzo Diagnostics, Life Technologies, Academic Press, Mueller Hinton, Specific Materials, The Netherlands, Easy Hyb, Image Pro Plus, Optional Materials, San Diego, Study Fruit Gene Transcription, Detect Methanotrophs Enriched, Detection of Malignant Plasma Cells, Genes Dev
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