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Gene Therapy of Cancer: Methods and Protocols (Methods in Molecular Biology)
 
 
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Gene Therapy of Cancer: Methods and Protocols (Methods in Molecular Biology) [Hardcover]

Wolfgang Walther (Editor), Ulrike S. Stein (Editor)

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Book Description

1934115851 978-1934115855 April 13, 2009 2nd ed.
Despite various difficulties, the field of gene therapy, particularly with regard to cancer, has accumulated a tremendous amount of vital pre-clinical and clinical data. "Gene Therapy of Cancer: Methods and Protocols, Second Edition" fully updates the first edition with expert coverage of established and novel protocols involving both experimental and clinical approaches to cancer gene therapy. This state-of-the-art volume contains overviews of new concepts and strategies with chapters on regulatory and ethical issues, developments, problems and possible limitations of design and production of gene therapeutics as well as translational issues. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Cutting edge and authoritative, "Gene Therapy of Cancer: Methods and Protocols, Second Edition" is an ideal guide for all those who wish to explore the fast-paced and critical study of nonviral, viral, experimental and clinical cancer gene therapy.

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Editorial Reviews

Review

From the reviews of the second edition: “This book, authored by a number of prominent researchers in the field … provides an overview of recent ground-breaking experimental and clinical concepts and protocols in cancer gene therapy. … the editors and authors are to be commended for producing a well-organised, authoritative and succinct coverage of the vast area of gene therapy protocols. The materials are relevant, easy-to-follow and well-presented with good illustrations. Without doubt it will serve as a useful resource for those interested in this field, both scientists and clinicians … .”­­­ (Han Hsi Wong and Yaohe Wang, Human Genetics, Vol. 128, July, 2010)

From the Back Cover

Despite various difficulties, the field of gene therapy, particularly with regard to cancer, has accumulated a tremendous amount of vital pre-clinical and clinical data. Gene Therapy of Cancer: Methods and Protocols, Second Edition fully updates the first edition with expert coverage of established and novel protocols involving both experimental and clinical approaches to cancer gene therapy. This state-of-the-art volume contains overviews of new concepts and strategies with chapters on regulatory and ethical issues, developments, problems and possible limitations of design and production of gene therapeutics as well as translational issues. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Cutting edge and authoritative, Gene Therapy of Cancer: Methods and Protocols, Second Edition is an ideal guide for all those who wish to explore the fast-paced and critical study of nonviral, viral, experimental and clinical cancer gene therapy.

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First Sentence:
The molecular definition of tumor antigens, costimulatory signals, and the possibility to genetically engineer tumor cells as well as simple protocols for efficient isolation and preparation of dendritic cells (DC) renew the interest in tumor immunotherapy and vaccination, in particular. Read the first page
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
ballistomagnetic vector system, ribozyme adenoviral vector, gliosarcoma cells, colony lift assay, autologous melanoma cells, herpes thymidine kinase genes, negative tumor cells, delay assay, gene gun immunization, rat antimouse, fluorescent fraction, cell strainer, ballistic transfer, tumor cell vaccines, producer cell line, cell bag, cytokine gene transfer, retroviral gene therapy, excision assay, anticancer prodrugs, human bladder carcinoma cells, suicide gene therapy, packaging cell line, master cell bank, cancer gene therapy
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Life Technologies, Cancer Res, Gene Ther, Molecular Medicine, Humana Press, New York, Boehringer Mannheim, Becton Dickinson, Cold Spring Harbor, San Diego, New England Biolabs, Acids Res, American Type Culture Collection, Cell Biol, Current Protocols, Nature Med, Perkin Elmer, Pharmacia Biotech, United States, Clinical Research Center, Dulbecco's Modified Eagle Medium, Fisher Scientific, Molecular Dynamics, Nature Genet, Palo Alto
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