This book describes techniques used in the study of transcription factors and DNA sequences involved in regulating gene transcription. The various methods used to analyze protein—DNA interactions such as electrophoretic mobility shift assay are described together with other more sophisticated footprinting techniques. Practical guidance is also provided on cloning and characterizing transcription factors and analyzing regulatory sequences, together with a description of database searching by homology. The major techniques employed in studying transcriptional regulation such as transcript analyses, transfection of DNA into cells and the use of reporter constructs are described in the companion volume to this book Gene Transcription: RNA Analysis. The Essential Techniques Series books are designed to provide you with immediate access to the protocols you require every day. These handy pocket-sized manuals are easy to carry around, and conveniently spiral bound making them ideal for lab bench work. Written by experienced laboratory researchers, each book in the Essential Techniques Series gives up-to-date, tried and tested practical information for the life scientist. For each key technique these books:
- introduce the most commonly used methods,
- explain the advantages and disadvantages of the methods, and give advice on which procedure to use,
- provide easy to follow step-by-step protocols, with experimental notes and tips on where to pause, plus information on safety and suppliers.
