This volume is designed to provide an easily accessible introduction to core strategies in the application of modern recombinant DNA technology. The first chapters directly address the applications of polymerase chain reaction to a variety of problems in DNA cloning that are, or have been, extremely challenging using more traditional approaches and technologies. These include cDNA cloning and transcript mapping, mutagenesis as well as the cloning of very long transcripts and protocols using limiting amounts of total RNA. Further chapters describe approaches to subtractive cloning technologies as well as novel specialized expression cloning and library screening strategies, such as variations on two-hybrid analysis for investigation of protein-protein interactions or investigation of protein-nucleic acid interactions.
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cell targeting ligands, megaprimer method, restriction digestion fragments, plaque amplification, individual phage clones, mutagenic primer, amplified phage, bait protein, mutant template, bait plasmid, color assay, packaging reaction, mutant fragment, bound phage, nuclease analysis, reporter strain, top agarose, plaque lifts, library construction, yeast transformation, upstream primers, vivo screening, ligase buffer, subtractive hybridization, competent cells Key Phrases - Capitalized Phrases (CAPs): (learn more)
Life Technologies, New York, Fisher Scientific, New England Biolabs, Nucleic Acids Res, Palo Alto, Proc Natl Acad Sci, Toll Free, Anal Biochem, Auburn University, Clontech Laboratories, Roest Crollius, Springer Lab Manual, Applied Biosystems, New Jersey, Small Quantities of Total, Amplification of Full-Length Enterovirus, Avenue Stanford, Fluorescent Hybridizations, High-Throughput Library Screening, Site Directed Deletion, Biosciences Clontech, Boehringer Mannheim, Cold Spring Harbor Laboratory Press, Corning Inc New!
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cell targeting ligands, megaprimer method, restriction digestion fragments, plaque amplification, individual phage clones, mutagenic primer, amplified phage, bait protein, mutant template, bait plasmid, color assay, packaging reaction, mutant fragment, bound phage, nuclease analysis, reporter strain, top agarose, plaque lifts, library construction, yeast transformation, upstream primers, vivo screening, ligase buffer, subtractive hybridization, competent cells Key Phrases - Capitalized Phrases (CAPs): (learn more)
Life Technologies, New York, Fisher Scientific, New England Biolabs, Nucleic Acids Res, Palo Alto, Proc Natl Acad Sci, Toll Free, Anal Biochem, Auburn University, Clontech Laboratories, Roest Crollius, Springer Lab Manual, Applied Biosystems, New Jersey, Small Quantities of Total, Amplification of Full-Length Enterovirus, Avenue Stanford, Fluorescent Hybridizations, High-Throughput Library Screening, Site Directed Deletion, Biosciences Clontech, Boehringer Mannheim, Cold Spring Harbor Laboratory Press, Corning Inc New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
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