A distinguished panel of international experts reviews the importance of glycobiology, an interdisciplinary field encompassing chemistry, biochemistry, biology, physiology, and pathology. Glycobiology Protocols highlights important methodological progress that has been made in the field of glycobiology, helping scientists to answer specific questions on glycoprotein structures, on the biosynthesis of glycoconjugates, and on the functions of lipid- or protein-bound carbohydrates. Glycobiology Protocols contains specific methods for the analysis of the structures or functions of glycoconjugates, as well as of glycosyltransferases and glycosidases involved in the biosynthesis of glycans. The methods described for specific systems can be modified for investigations of similar biomolecules and tissues or cell types. Comprehensive and highly practical, Glycobiology Protocols provides researchers with a solid basis for studying the many aspects of glycobiology with state-of-the-art methods. The protocols follow the successful Methods in Molecular Biology™ series format, with each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
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"...a very valuable handbook for a wide audience of specialists involved in studies of carbohydrates and carbohydrate-containing biopolymers; glycobiologists, biochemists, bioorganic chemists, and molecular and cell biologists." -Biochemistry
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First Sentence:
In the lumenal space of the endoplasmic reticulum (ER), asparagine (N)-linked glycoproteins are formed by en bloc transfer of preformed oligosaccharide (OS) units from lipid-linked oligosaccharide (LLO) donors to nascent polypeptides with asparaginyl residues in the context Asn-X-Ser/Thr (see refs. 1 and 2; see also Chapters 2 and 3). Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
mucin standard, mono gel, pertrimethylsilylated derivatives, anionic glycoconjugates, bone cancer cells, occurring sialic acids, luminal segment, dissociation curve analysis, sulfogalactosyl ceramide, aggregation suppression, dextran ladder, gel stock solution, stacking buffer, zona matrix, carbohydrate electrophoresis, selectin function, sialidase activity, released glycans, thrombospondin type, mouse zona pellucida, globotriaosyl ceramide, human osteosarcoma cells, primary osteoblasts, diagnostic ions, methyl ester derivatives Key Phrases - Capitalized Phrases (CAPs): (learn more)
Glycobiology Protocols Edited, Humana Press Inc, Key Words, Structures of Naturally Occurring Sialic Acids, Reactive Yellow, Fisher Scientific, Methods Mol, Structural Analysis of N-Glycans, Applied Biosystems, Cell Biol, Partial Purification of Man-P-Undec, Perkin Elmer, Nonradioactive Trans-Sialidase Screening Assay, Structural Determination of O-Glycans, The Netherlands, Amersham Biosciences, Analysis of Mucins, Becton Dickinson, American Radiolabeled Chemicals, Molecular Dissection of Mouse Zona Pellucida, Molecular Probes, Nucleic Acids Res, Primers Ref, Roche Diagnostics, Synthase Assay New!
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Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
In the lumenal space of the endoplasmic reticulum (ER), asparagine (N)-linked glycoproteins are formed by en bloc transfer of preformed oligosaccharide (OS) units from lipid-linked oligosaccharide (LLO) donors to nascent polypeptides with asparaginyl residues in the context Asn-X-Ser/Thr (see refs. 1 and 2; see also Chapters 2 and 3). Read the first page Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
mucin standard, mono gel, pertrimethylsilylated derivatives, anionic glycoconjugates, bone cancer cells, occurring sialic acids, luminal segment, dissociation curve analysis, sulfogalactosyl ceramide, aggregation suppression, dextran ladder, gel stock solution, stacking buffer, zona matrix, carbohydrate electrophoresis, selectin function, sialidase activity, released glycans, thrombospondin type, mouse zona pellucida, globotriaosyl ceramide, human osteosarcoma cells, primary osteoblasts, diagnostic ions, methyl ester derivatives Key Phrases - Capitalized Phrases (CAPs): (learn more)
Glycobiology Protocols Edited, Humana Press Inc, Key Words, Structures of Naturally Occurring Sialic Acids, Reactive Yellow, Fisher Scientific, Methods Mol, Structural Analysis of N-Glycans, Applied Biosystems, Cell Biol, Partial Purification of Man-P-Undec, Perkin Elmer, Nonradioactive Trans-Sialidase Screening Assay, Structural Determination of O-Glycans, The Netherlands, Amersham Biosciences, Analysis of Mucins, Becton Dickinson, American Radiolabeled Chemicals, Molecular Dissection of Mouse Zona Pellucida, Molecular Probes, Nucleic Acids Res, Primers Ref, Roche Diagnostics, Synthase Assay New!
Books on Related Topics | Concordance | Text Stats Browse Sample Pages:
Front Cover | Table of Contents | First Pages | Index | Back Cover | Surprise Me!
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