Green Fluorescent Protein: Applications & Protocols (Methods in Molecular Biology) [Hardcover]

Barry W. Hicks (Editor)

Book Description

ISBN-10: 0896039056 | ISBN-13: 978-0896039056 | Publication Date: June 1, 2002 | Edition: 1st

Barry W. Hicks and a team of well-practiced experimentalists demonstrate the power and the versatility of the green fluorescent protein (GFP) marker with a cutting-edge collection of readily reproducible GFP techniques. These include methods for detecting and imaging GFP (FRET, BRET, and whole-body imaging); for using GFP to create and select for transgenic organisms, for its use as a biosensor; and for studying viruses.

Editorial Reviews

Review

"The breadth of applications for green fluorescent protein will make this book useful for many laboratories and may provide new approaches for others not previously familiar with gfp as a tool. Gfp has provided a wide ranging and valuable tool for many investigators. This is a very useful set of protocols, even for those who don't think they have an application for the technique. It is especially valuable to have the diverse methods compiled in a single book. Most will find something of value." -Doody's Health Sciences Book Review Journal "...this book truly is a collection of "Applications and protocols" and such is the degree of detail in the methods described that it should perhaps have been sub-titled "A Laboratory Manual." -The Analyst

From the Back Cover

Green fluorescent protein (GFP), a critically important marker protein, allows direct visualization in live samples without the aid of additional sample preparation. In Green Fluorescent Protein: Applications and Protocols, Barry W. Hicks and a team of well-practiced experimentalists demonstrate the power and the versatility of this marker with a cutting-edge collection of readily reproducible GFP techniques. These include methods for detecting and imaging GFP (FRET, BRET, and whole-body imaging), for using GFP to create and select for transgenic organisms, for its use as a biosensor, and for studying viruses. Each method is described in step-by-step detail to ensure robust results and can be used in applications ranging from agriculture and botany to human gene therapy. Extensive notes review the materials required, explain the scientific basis of each step and possible sources of error, and provide tips for overcoming problems. A valuable companion CD-ROM displays many color figures and videos from over half the chapters. Highly practical and state-of-the-art, Green Fluorescent Protein: Applications and Protocols makes available to all biological and biomedical experimentalists a full array of the powerful new GFP techniques.

Product Details

• Hardcover: 408 pages
• Publisher: Springer; 1st edition (June 1, 2002)
• Language: English
• ISBN-10: 0896039056
• ISBN-13: 978-0896039056
• Product Dimensions: 9.1 x 6.3 x 0.9 inches
• Shipping Weight: 1.7 pounds (View shipping rates and policies)
• Amazon Best Sellers Rank: #644,002 in Books (See Top 100 in Books)