Green Fluorescent Proteins, Volume 58 (Methods in Cell Biology, Volume 58) [Paperback]

Kevin F. Sullivan (Editor), Steve A. Kay (Editor), Leslie Wilson (Series Editor), Paul T. Matsudaira (Series Editor)

Book Description

ISBN-10: 0126760756 | ISBN-13: 978-0126760750 | Publication Date: October 28, 1998 | Edition: 1

This volume is an authoritative and comprehensive treatment of the approaches and techniques used for Green Fluorescent Proteins (GFP). The primary focus of this work is on research using biological systems. The volume covers all aspects of GFP, from its expression in different organisms to specific microscopic and data analysis methods.

Key Features
* Only volume on Green Fluorescent Protein research
* Covers all aspects of GFP
* Provides specific microscopic and data analysis methods
* Discusses the design and construction of GFP fusion proteins
* Covers GFP expression in animals, insects, plants, and microbes
* Details procedures for time lapse imaging of living cells
* Explains how to implement single molecule fluorescence detection with GFP
* Discusses dual label GFP strategies for multicolor fluorescence
* Presents fluorescence resonance energy transfer methods with GFPs
* Details quantitative fluorescence imaging techniques
* Extensively illustrated with color photographs

Editorial Reviews

From the Back Cover

The green fluorescent Protein (GFP) has initiated a revolution in molecular cell biology by establishing a general approach for visualizing nearly any protein of interest in any cell, tissue or species. Researchers working at all levels of biology, such as single molecule dynamics, protein trafficking within cells, organelle dynamics and cell and tissue behaviors during development, have made important use of GFP.
This volume is a practical guide to working with GFP and contains discussions of GFP biophysics and GFP mutants, design of GFP fusion proteins and expression systems, and detailed protocols for introducing and visualizing GFP in living cells. Methods for quantitative imaging, data analysis and presentation are detailed by a select group of researchers who have made some of the key contributions to bio-fluorescent imaging technologies.
Key Features
* Discusses the design and construction of GFP fusion proteins
* Covers GFP expression in animals, insects, plants, and microbes
* Details procedures for time-lapse imaging of living cells
* Explains how to implement single-molecule fluorescence detection with GFP
* Discusses dual-label GFP strategies for multicolor fluorescence
* Presents fluorescence resonance energy transfer methods with GFPs
* Details quantitative flourescence imagine techniques
* Extensively illustrated with color photographs

About the Author

Edited by Leslie I. Wilson, Paul T. Matsudaira, Kevin F. Sullivan, and SteveA. Kay --This text refers to an out of print or unavailable edition of this title.

Product Details

• Paperback: 386 pages
• Publisher: Academic Press; 1 edition (October 28, 1998)
• Language: English
• ISBN-10: 0126760756
• ISBN-13: 978-0126760750
• Product Dimensions: 9.3 x 7.8 x 1 inches
• Shipping Weight: 1.8 pounds
• Average Customer Review: 5.0 out of 5 stars  See all reviews (1 customer review)
• Amazon Best Sellers Rank: #2,458,956 in Books (See Top 100 in Books)

Customer Reviews

Most Helpful Customer Reviews

5 of 6 people found the following review helpful:

5.0 out of 5 stars Excellent introduction to GFP, November 28, 1999

By A Customer

This review is from: Green Fluorescent Proteins, Volume 58 (Methods in Cell Biology, Volume 58) (Paperback)

This book is really interesting to read, it covers many types of applications of GFP, and is also packed with nice pictures that make it fun to read. Lots of helpful hints too. Highly recommended to anyone who is thinking of using GFP. This book would be useful both to those just starting to use GFP as well as experienced researchers looking to see what others have tried.