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Guide to Protein Purification, Volume 182: Volume 182: Guide to Protein Purification (Methods in Enzymology)
 
 
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Guide to Protein Purification, Volume 182: Volume 182: Guide to Protein Purification (Methods in Enzymology) [Paperback]

John N. Abelson (Editor), Melvin I. Simon (Editor), Murray P. Deutscher (Editor)
5.0 out of 5 stars  See all reviews (2 customer reviews)


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Book Description

0122135857 978-0122135859 March 14, 1990
The Guide to Protein Purification is a self-contained volume covering all the important procedures for purifying proteins, as well as other more specialized techniques. However, to stay within the confines of a single volume, some details are dealt with by reference to other works, but these have been kept to a minimum. It is hoped that this volume will satisfy the needs of both the novice in protein purification and the more experienced researcher.

Key Features
* Contains procedures newly written for this volume
* Provides extensive practical information
* Clearly describes rationale and strategies for protein and enzyme purification
* Offers personal perspectives on enzyme purification by eminent researchers

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Editorial Reviews

Review

"In summary, this is possibly the most important, comprehensive and affordable work on protein purification to have appeared in recent years."
TIB-TECH

From the Back Cover

Guide to Protein Purification, designed to serve the needs of the student, experienced researcher, and newcomer to the field is a comprehensive manual that presents in one source, all up-to-date procedures necessary for purifying, characterizing, and handling proteins and enzymes.
Key features of the guide:
* Detailed procedures newly written for this volume
* Extensive practical information
* Rationale and strategies for protein and enzyme purification
* Personal perspectives on enzyme purification by eminent researchers
Among the topics covered:
* General methods for handling proteins and enzymes
* Extraction, subcellular fractionation, and solubilization procedures
* Comprehensive purification techniques
* Specialized purification procedures
* Protein characterization
* Immunological procedures
* Computer analysis of protein structure

Product Details

  • Paperback: 894 pages
  • Publisher: Academic Press (March 14, 1990)
  • Language: English
  • ISBN-10: 0122135857
  • ISBN-13: 978-0122135859
  • Product Dimensions: 9.7 x 7.5 x 1.7 inches
  • Shipping Weight: 3.1 pounds
  • Average Customer Review: 5.0 out of 5 stars  See all reviews (2 customer reviews)
  • Amazon Best Sellers Rank: #1,910,102 in Books (See Top 100 in Books)

 

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5.0 out of 5 stars EXCELLENT BOOK, May 30, 2010
This review is from: Guide to Protein Purification, Volume 182: Volume 182: Guide to Protein Purification (Methods in Enzymology) (Paperback)
Very Useful Guide to Protein Work. Excellent wrapping and care delivered on time. Clean item as descriped. Thank you.
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3 of 7 people found the following review helpful:
5.0 out of 5 stars A must book for biochemist that intend to purify proteins, June 13, 2000
By A Customer
This review is from: Guide to Protein Purification, Volume 182: Volume 182: Guide to Protein Purification (Methods in Enzymology) (Paperback)
This is a must book for biochemist and people that intend to become one. It gives a vast background on most biochemical methods used for the purification of proteins, including the history behind the methods. Not to mention the availability of comments on most topics, that help in understading the limits of the various procedures.
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Inside This Book (learn more)
First Sentence:
"Don't waste clean thinking on dirty enzymes" is an admonition of Efraim Racker's which is at the core of enzymology and good chemical practice. Read the first page
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
radiolabeled cell extracts, aminoacyl transfer reaction, immobilized dye chromatography, immobilized dye column, unlabeled cell extracts, silver stain protocols, diamine stains, maximizing gene expression, radioactive antigen, bisacrylamide stock, immobilized reactive dyes, unbound iodine, gradient mix, bicyclic cascade, iodination mixture, immobilized dyes, start buffer, solubilization agent, iodinated protein, solubilization buffer, chromatographic solvent, desired protein, solubilization conditions, search matrix, electrode buffer
Key Phrases - Capitalized Phrases (CAPs): (learn more)
New York, Cell Biol, Nucleic Acids Res, Cold Spring Harbor, Bead Beater, Bromphenol Blue, New Jersey, Coomassie Brilliant Blue, Protein Chem, Amido Black, Practical Approach, San Francisco, Bethesda Research Laboratories, Fast Green, Los Angeles, Two-Dimensional Gel Electrophoresis of Proteins, Van Keuren, Boca Raton, Colloid Interface Sci, Crocein Scarlet, Humana Press, Methods of Enzymatic Analysis, Palo Alto, Pierce Apparatus Branch, Protein Eng
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