Detergents can enter water and wastewater by discharge of domestic and industrial cleansing waters. The most widely used detergents are linear alkyl sulfonates (LAS) and alkyl benzene sulfonates (ABS): LAS are preferable to ABS because they are biodegradable, thus readily decomposed by microorganismicrosecond. The presence of anionic LAS/ABS detergents in natural waters should be below 0.1 micrograms per litre and in raw domestic wastewater in the range from 1 to 20 micrograms per litre. The aqueous solution containing the detergents is treated with the colored indicator. The reaction product can be extracted in the chloroform layer, while the original dyestuff is insoluble in the organic medium. The intensity of the color developed is proportional to the concentration of the detergents present. Kit comes with 15mL detergents reagent A, 15mL detergent reagent B, 180mL chloroform, demineralizer bottle with filter cap for 12 L, checker disc, 30mL long glass vials with caps (2), long plastic pipette, 3mL plastic pipette and 1mL plastic pipette. Checker disc method. 0.00-1.30 micrograms per litre Range. 0.02 micrograms per litre Smallest increment. Methylene blue chemical method. 35 Tests. 1245 grams.