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Helicobacter Pylori Protocols (Methods in Molecular Medicine)
 
 
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Helicobacter Pylori Protocols (Methods in Molecular Medicine) [Hardcover]

Christopher L. Clayton (Editor), Harry L. T. Mobley (Editor)

Price: $125.00 & this item ships for FREE with Super Saver Shipping. Details
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Book Description

Methods in Molecular Medicine April 15, 1997
Helicobacter pylori Protocols offers an outstanding collection of state-of-the-art protocols for the identification and molecular manipulation of H. pylori. The authoritative contributors supply detailed and readily reproducible protocols for the culturing of H. pylori, for the isolation and restriction endonuclease digestion of H. pylori chromosomal DNA, and for the transformation and insertional mutagenesis of H. pylori. They also provide molecular epidemiological techniques, including ribotyping, PCR-RFLP, and RAPD-PCR. These procedures have been developed by leading practitioners to solve the difficult technical problems created by the application of the powerful bacterial genetic and molecular cloning techniques to H. pylori.

Editorial Reviews

Review

"The book was designed to provide up-to-date detailed protocols for the experienced either working in the field of Helicobacter research or wishing to enter the field. The authors share their experience and give advice about how to avoid pitfalls and problems encountered with this organism. The book is recommended for those with a serious interest in microbiology and molecular biology of Helicobacter pylori. - David Graham, MD, Baylor College of Medicine

From the Back Cover

In Helicobacter pylori Protocols, leading experts bring together an outstanding collection state-of-the-art protocols for the identification and molecular manipulation of H. pylori. The authoritative contributors supply detailed and readily reproducible protocols for culturing H. pylori, for the isolation and restriction endonuclease digestion of H. pylori chromosomal DNA, and for the transformation and insertional mutagenesis of H. pylori. They also provide molecular epidemiological techniques, including ribotyping, PCR-RFLP, and RAPD-PCR. These procedures have been developed to solve the difficult technical problems created by the application of today's powerful bacterial genetic and molecular cloning techniques to H. pylori. Helicobacter pylori Protocols contains a wide variety of stand-alone techniques that are unfailingly reproducible. The book is certain to become a standard experimental and clinical laboratory reference for all those engaged in H. pylori work today.

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Inside This Book (learn more)
First Sentence:
Until the discovery of Helicobacter pylori in 1982, the normal human stomach was generally considered to be sterile, or transiently populated by oropharyngeal bacteria carried there by peristalsis. Read the first page
Key Phrases - Statistically Improbable Phrases (SIPs): (learn more)
pylori cells, biopsy urease test, gastritis scores, microbiological processing, pylori genes, pylori isolates, brucella broth, pylori strains, microaerobic atmosphere, cytotoxin activity, chronic superficial gastritis, fresh clinical isolates, pylori growth, pylori genome, gene restriction patterns, superficial epithelial cells, challenge inoculum, digest analysis, microaerobic conditions, cytotoxin production, derivative plasmid, endoscopy unit, gel tray, gnotobiotic piglets, pylori infection
Key Phrases - Capitalized Phrases (CAPs): (learn more)
Boehringer Mannheim, Mobley Humana Press Inc, Molecular Medicine, Protocols Edited, New York, Life Technologies, Nucleic Acids Res, Becton Dickinson, Receptor Characterization, Introduction Helicobacter, Agents Chemother, Cold Spring Harbor Laboratory, Detecting Ulcerogenic Strains, Genomic Library, Laboratory Manual, Study of Metabolism, Vector Containing, Characteristics of Helicobacter, Current Protocols, Perkin-Elmer Cetus, San Francisco
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