"Many leading researchers in the field are included in the book. . .It describes state-of-the-art methods and tools that are being used to address important medical and scientific questions. . .not only a highly practical manual for people working in laboratory medicine. . .also valuable for virologists, gastroenterologists, hepatologists and other medical infectious disease specialists."-Infection "The useful clinical methods sections include description of methods for the detection of antibodies to HCV, quantitative HCV serum RNA assays, polymerase chain reaction (PCR) assays for the detection of HCV in liver and other tissues, HCV genotyping methods, and detection of HCV by in situ hybridization....Additional sections describe methods used by research, development, and production laboratories to clone and express HCV genes, to study viral replication and viral protein function, to produce recombinant HCV proteins, and to create in vitro culture models to allow study of viral replication and possible anitiviral compounds....The "Notes" sections will be particularly useful to individuals setting up the method for the first time. All the chapters contain current information and are well written....the scope of the book of use to anyone desiring to expand his or her knowledge of HCV. The introductory material for the sections is the best compilation of HCV-related information available in print today and makes Hepatitis C Protocols a valuable addition to the published literature on HCV."-Laboratory Medicine "In summary Johnson Yiu-Nam Lau should be applauded for assembling an outstanding international ensemble of experts to produce a truly authoritative handbook for HCV clinicians and researchers."-The Quarterly Review of Biology ". . . an excellent review of the current and most up-to-date methods in molecular medicine involving hepatitis C. . . clinicians should review this textbook to give them a better understanding of the molecular techniques and shortcomings of the various diagnostic assays that they use in their day to day practice."-Canadian Journal of Infectious Disease
From the Back Cover
In Hepatitis C Protocols, Johnson YN Lau and his expert collaborators present cutting-edge techniques for study of the hepatitis C virus infection and its disease, hepatitis C, which afflict an estimated 170 million people worldwide. The methods range from the detection of anti-HCV in serum and HCV in liver tissue, to HCV genotyping and the detection and quantification of HCV in serum. In addition to these key protocols, the authoritative contributors also evaluate recombinant proteins in immunological test systems, including the identification of important antigenic epitopes, antibodies, and recombinant antigens. Molecular and immunological techniques to study this virus and its infection, as well as animal models and recombinant virus technology commonly used to express hepatitis C viral proteins, are also developed. Hepatitis C Protocols summarizes the state-of-the-art in today's research and understanding of the virology of hepatitis C virus. The methods and systems detailed in this book will play a key role in the development of more effective antivirals, in the development of vaccines, and in a deeper understanding of viral replication strategies, viron structure, and the mechanisms of viron assembly and release. A valuable new standard resource for all researchers and clinicians, Hepatitis C Protocols leads the way toward improved prevention, diagnosis, and treatment of viral hepatitis C.