High Resolution Chromatography: A Practical Approach [Hardcover]

Paul Millner (Editor)

Book Description

Publication Date: July 10, 1999 | ISBN-10: 0199636494 | ISBN-13: 978-0199636495 | Edition: 1

Isolating and purifying proteins is a key step for a wide range biological experiments, and researchers require quick and reliable techniques. This practical volume fits this need. The first section, devoted to techniques and equipment, covers initial preparation, microscale techniques, detector technologies, and capillary electrophoresis and its associated techniques. The second section then covers a range of chromatographic procedures based on the interaction of a specific ligand with its target protein or other macromolecule. Some chapters cover non-specific interactions using peptides, inhibitors, and antibodies as the affinity ligand, while others focus on specific groups of molecules, including oligosaccharides and glycosylated proteins, nucleotide-binding proteins, proteins binding free and chelated metal ions, and DNA-binding proteins.

Editorial Reviews

Review

"Laboratory workers will find this a useful guide to the development of effective purification protocols" Aslib Book Guide"

About the Author

Paul Millner is at University of Leeds.

Product Details

• Hardcover: 336 pages
• Publisher: Oxford University Press, USA; 1 edition (July 10, 1999)
• Language: English
• ISBN-10: 0199636494
• ISBN-13: 978-0199636495
• Product Dimensions: 9.3 x 6.2 x 0.9 inches
• Shipping Weight: 1.5 pounds (View shipping rates and policies)
• Amazon Best Sellers Rank: #10,763,521 in Books (See Top 100 in Books)