This book describes the key procedures for obtaining and staining chromosomes from a variety of human tissues. Techniques for the culture of amniotic fluid, chorionic villi, blood lymphocytes, bone marrow and lymph node cells are all described in detail. Reliable methods used for staining and banding are provided together with an overview of the modern fluorescence in situ hybridization (FISH) technology which complements the traditional approaches. This is an essential practical guide for all workers in cytogenetics. Its authoritative coverage is invaluable both for newcomers and more experienced colleagues in the field. The Essential Techniques Series books are designed to provide you with immediate access to the protocols you require every day. These handy pocket-sized manuals are easy to carry around, and conveniently spiral bound making them ideal for lab bench work. Written by experienced laboratory researchers, each book in the Essential Techniques Series gives up-to-date, tried and tested practical information for the life scientist. For each key technique these books:
- introduce the most commonly used methods,
- explain the advantages and disadvantages of the methods, and give advice on which procedure to use,
- provide easy to follow step-by-step protocols, with experimental notes and tips on where to pause, plus information on safety and suppliers.
